A1 Refereed original research article in a scientific journal
Histamine metabolism and transport are deranged in human keratinocytes in oral lichen planus
Authors: Salem A, Rozov S, Al-Samadi A, Stegajev V, Listyarifah D, Kouri VP, Han X, Nordström D, Hagström J, Eklund KK
Publication year: 2017
Journal: British Journal of Dermatology
Journal name in source: The British journal of dermatology
Journal acronym: Br J Dermatol
Volume: 176
Issue: 5
First page : 1213
Last page: 1223
ISSN: 0007-0963
eISSN: 1365-2133
DOI: https://doi.org/10.1111/bjd.14995
Abstract
R pattern, which is deranged in oral lichen planus (OLP).\nTo investigate histamine metabolism and transport in HOKs of healthy controls and patients with OLP.\nTissue sections and cultured primary HOKs were studied using immunostaining, quantitative real-time polymerase chain reaction and confocal microscopy. Histamine levels were analysed using high-performance liquid chromatography.\nl-histidine decarboxylase (HDC) and organic cation transporter (OCT)3 were increased in mRNA and protein levels in patients with OLP compared with controls. In contrast, histamine N-methyltransferase (HNMT) immunoreactivity was decreased in OLP. OCT1/OCT2 and diamine oxidase were not detectable in either tissue sections or in HOKs. Immunolocalization of HDC and OCT3 in HOKs revealed moderate-to-high expression within cytoplasm and cell boundaries. Stimulation with lipopolysaccharide (LPS) or interferon-γ upregulated HDC-gene transcript in HOKs, whereas this was downregulated with high histamine concentration and tumour necrosis factor-α. LPS induced a dose-dependent release of low histamine in HOKs, while high histamine concentration downregulated epithelial adhesion proteins.\n R in autocrine and paracrine modes. The substantially deranged histamine metabolism and transport in OLP could, in part, contribute to the disease pathogenesis.\nBACKGROUND\nOBJECTIVES\nMETHODS\nRESULTS\nCONCLUSIONS
R pattern, which is deranged in oral lichen planus (OLP).\nTo investigate histamine metabolism and transport in HOKs of healthy controls and patients with OLP.\nTissue sections and cultured primary HOKs were studied using immunostaining, quantitative real-time polymerase chain reaction and confocal microscopy. Histamine levels were analysed using high-performance liquid chromatography.\nl-histidine decarboxylase (HDC) and organic cation transporter (OCT)3 were increased in mRNA and protein levels in patients with OLP compared with controls. In contrast, histamine N-methyltransferase (HNMT) immunoreactivity was decreased in OLP. OCT1/OCT2 and diamine oxidase were not detectable in either tissue sections or in HOKs. Immunolocalization of HDC and OCT3 in HOKs revealed moderate-to-high expression within cytoplasm and cell boundaries. Stimulation with lipopolysaccharide (LPS) or interferon-γ upregulated HDC-gene transcript in HOKs, whereas this was downregulated with high histamine concentration and tumour necrosis factor-α. LPS induced a dose-dependent release of low histamine in HOKs, while high histamine concentration downregulated epithelial adhesion proteins.\n R in autocrine and paracrine modes. The substantially deranged histamine metabolism and transport in OLP could, in part, contribute to the disease pathogenesis.\nBACKGROUND\nOBJECTIVES\nMETHODS\nRESULTS\nCONCLUSIONS
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