Refereed journal article or data article (A1)
Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes
List of Authors: Prabhakar Neeraj, Belevich Ilya, Peurla Markus, Heiligenstein Xavier, Chang Huan-Cheng, Sahlgren Cecilia, Jokitalo Eija, Rosenholm Jessica M
Publisher: MDPI
Publication year: 2021
Journal: Nanomaterials
Journal name in source: NANOMATERIALS
Journal acronym: NANOMATERIALS-BASEL
Volume number: 11
Issue number: 1
Number of pages: 10
eISSN: 2079-4991
DOI: http://dx.doi.org/10.3390/nano11010014
URL: https://www.mdpi.com/2079-4991/11/1/14
Self-archived copy’s web address: https://research.utu.fi/converis/portal/Publication/53003270
Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets.
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