Vertaisarvioitu alkuperäisartikkeli tai data-artikkeli tieteellisessä aikakauslehdessä (A1)
Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes
Julkaisun tekijät: Prabhakar Neeraj, Belevich Ilya, Peurla Markus, Heiligenstein Xavier, Chang Huan-Cheng, Sahlgren Cecilia, Jokitalo Eija, Rosenholm Jessica M
Kustantaja: MDPI
Julkaisuvuosi: 2021
Journal: Nanomaterials
Tietokannassa oleva lehden nimi: NANOMATERIALS
Lehden akronyymi: NANOMATERIALS-BASEL
Volyymi: 11
Julkaisunumero: 1
Sivujen määrä: 10
eISSN: 2079-4991
DOI: http://dx.doi.org/10.3390/nano11010014
Verkko-osoite: https://www.mdpi.com/2079-4991/11/1/14
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/Publication/53003270
Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
