A1 Refereed original research article in a scientific journal
Selective Gelatinase Inhibitor Peptide Is Effective in Targeting Tongue Carcinoma Cell Tumors In Vivo
Authors: Suojanen Juho, Vilen Suvi-Tuuli, Nyberg Pia, Heikkilä Pia, Penate-Medina Oula, Saris Per EJ, Hagström J, Ranta Tanja-Maria, Salo Tuula, Sorsa Timo, Reunanen Justus
Publisher: INT INST ANTICANCER RESEARCH
Publication year: 2011
Journal: Anticancer Research
Journal name in source: ANTICANCER RESEARCH
Journal acronym: ANTICANCER RES
Volume: 31
Issue: 11
First page : 3659
Last page: 3664
Number of pages: 6
ISSN: 0250-7005
Abstract
Background: Matrix metalloproteinases (MMP) are strongly associated with cancer progession. Broad-spectrum MMP inhibition is rarely beneficial clinically due to adverse effects. Of all MMPs, the gelatinases are associated with the spread of several types of cancer, including oral carcinoma. We have developed gelatinase-specific peptides, as well as their fusion with green fluorescent protein (GFP), capable of effectively targeting carcinomas. Materials and Methods: Effects on tumor growth and lymphatic micrometastatic spread in vivo was studied by use of HSC-3-cell xenografted athymic nude mice. Antigelatinolytic mono- vs. polytherapies, as well as biological activity of peptide-GFP fusion, were also analyzed in vivo. Results: Antigelatinolytic therapy effectively inhibited,growth of xenografted tumors in mice but the proportion of enlarged lymph nodes remained the same; antigelatinolytic polytherapy seemed not to potentiate the antitumor effects. The peptide GFP chimera sustained its activity in vivo and effectively homed to the primary tumors. Conclusion: Peptide gelatinase inhibitors are effective in inhibiting primary tumor growth but alone do not prevent the spread of carcinoma cells; however, their bioactive GFP fusion is a candidate for tumor characterization and imaging.
Background: Matrix metalloproteinases (MMP) are strongly associated with cancer progession. Broad-spectrum MMP inhibition is rarely beneficial clinically due to adverse effects. Of all MMPs, the gelatinases are associated with the spread of several types of cancer, including oral carcinoma. We have developed gelatinase-specific peptides, as well as their fusion with green fluorescent protein (GFP), capable of effectively targeting carcinomas. Materials and Methods: Effects on tumor growth and lymphatic micrometastatic spread in vivo was studied by use of HSC-3-cell xenografted athymic nude mice. Antigelatinolytic mono- vs. polytherapies, as well as biological activity of peptide-GFP fusion, were also analyzed in vivo. Results: Antigelatinolytic therapy effectively inhibited,growth of xenografted tumors in mice but the proportion of enlarged lymph nodes remained the same; antigelatinolytic polytherapy seemed not to potentiate the antitumor effects. The peptide GFP chimera sustained its activity in vivo and effectively homed to the primary tumors. Conclusion: Peptide gelatinase inhibitors are effective in inhibiting primary tumor growth but alone do not prevent the spread of carcinoma cells; however, their bioactive GFP fusion is a candidate for tumor characterization and imaging.
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