A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

Betaine supplementation causes increase in carnitine metabolites in the muscle and liver of mice fed a high-fat diet as studied by nontargeted LC-MS metabolomics approach




TekijätPekkinen J, Olli K, Huotari A, Tiihonen K, Keski-Rahkonen P, Lehtonen M, Auriola S, Kolehmainen M, Mykkänen H, Poutanen K, Hanhineva K

Julkaisuvuosi2013

JournalMolecular Nutrition and Food Research

Tietokannassa oleva lehden nimiMolecular nutrition & food research

Lehden akronyymiMol Nutr Food Res

Vuosikerta57

Numero11

Aloitussivu1959

Lopetussivu68

Sivujen määrä10

ISSN1613-4125

eISSN1613-4133

DOIhttps://doi.org/10.1002/mnfr.201300142


Tiivistelmä
Betaine (BET) reduces diet-induced liver lipid accumulation, and may relieve obesity-related metabolic disturbances. The aim of our study was to analyze metabolite alterations after supplementation of BET, polydextrose (PDX, a soluble dietary fiber), or their combination (BET PDX) via drinking water to C57BL/6J mice fed a high-fat (HF) diet.\nBET supplementation increased BET levels in plasma, muscle, and liver (p < 0.05), and the nontargeted LC-MS metabolite profiling revealed an increase in several metabolites in the carnitine biosynthesis pathway after BET supplementation both in liver and muscle. These included carnitine and acetylcarnitine (1.4-fold, p < 0.05), propionylcarnitine and γ-butyrobetaine (1.5-fold, p < 0.05), and several other short-chain acylcarnitines (p < 0.05) in muscle. These changes were slightly higher in the BET PDX group. Furthermore, BET reduced the HF diet induced accumulation of triglycerides in liver (p < 0.05). The supplementations did not attenuate the HF diet induced increase in body weight gain or the increase in adipose tissue mass. Instead, the combination of BET and PDX tended to increase adiposity.\nOur results suggest that increased availability of BET in different tissues, especially in muscle, after BET supplementation has an impact on carnitine metabolism, and this could further explain the link between BET and lipid metabolism.\nSCOPE\nMETHODS AND RESULTS\nCONCLUSION



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