A1 Refereed original research article in a scientific journal
ALTERED COLLAGEN EXPRESSION IN HUMAN DENTIN - INCREASED REACTIVITY OF TYPE-III AND PRESENCE OF TYPE-VI IN DENTINOGENESIS IMPERFECTA, AS REVEALED BY IMMUNOELECTRON MICROSCOPY
Authors: WALTIMO J, RISTELI L, RISTELI J, LUKINMAA PL
Publisher: SAGE PUBLICATIONS LTD
Publication year: 1994
Journal: Journal of Histochemistry and Cytochemistry
Journal name in source: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Journal acronym: J HISTOCHEM CYTOCHEM
Volume: 42
Issue: 12
First page : 1593
Last page: 1601
Number of pages: 9
ISSN: 0022-1554
DOI: https://doi.org/10.1177/42.12.7983359
Abstract
We used transmission immunoelectron microscopy and polyclonal antibodies to study the reactivities of Types III and VI collagen in dentin of normal human permanent and primary teeth and in primary teeth from five patients with dentinogenesis imperfecta (DI) associated with osteogenesis imperfecta and occurring as a single trait. In the normal permanent tooth, reactivity of Type III collagen was occasional and, where present, peritubular. Staining of normal primary teeth was less occasional but still rare, whereas the abnormal dentin stained more uniformly. Atypical, nonstriated fibrillar structures that also showed Type III collagen reactivity were observed in dentin of two of the three patients with DI as a single trait. Later, these two patients proved co be fist cousins, Unlike antibodies to the N-terminal pro-peptide of Type I pro-collagen, antibodies to the C-terminal telopeptide of Type I collagen, used for comparison stained the affected dentin homogeneously. Reactivity of Type Vi collagen, not detected in normal teeth, was seen in the dentin of all abnormal teeth, in association with non-fibrillar delicate material. This study also shows that although readily detectable in dentin affected by DI, Type III collagen is a minor constituent of normal human dentin matrix.
We used transmission immunoelectron microscopy and polyclonal antibodies to study the reactivities of Types III and VI collagen in dentin of normal human permanent and primary teeth and in primary teeth from five patients with dentinogenesis imperfecta (DI) associated with osteogenesis imperfecta and occurring as a single trait. In the normal permanent tooth, reactivity of Type III collagen was occasional and, where present, peritubular. Staining of normal primary teeth was less occasional but still rare, whereas the abnormal dentin stained more uniformly. Atypical, nonstriated fibrillar structures that also showed Type III collagen reactivity were observed in dentin of two of the three patients with DI as a single trait. Later, these two patients proved co be fist cousins, Unlike antibodies to the N-terminal pro-peptide of Type I pro-collagen, antibodies to the C-terminal telopeptide of Type I collagen, used for comparison stained the affected dentin homogeneously. Reactivity of Type Vi collagen, not detected in normal teeth, was seen in the dentin of all abnormal teeth, in association with non-fibrillar delicate material. This study also shows that although readily detectable in dentin affected by DI, Type III collagen is a minor constituent of normal human dentin matrix.
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