Vertaisarvioitu alkuperäisartikkeli tai data-artikkeli tieteellisessä aikakauslehdessä (A1)

Rapid Transcriptional Reprogramming Triggered by Alteration of the Carbon/Nitrogen Balance Has an Impact on Energy Metabolism in Nostoc sp. PCC 7120




Julkaisun tekijät: Gollan Peter J., Muth-Pawlak Dorota, Aro Eva-Mari

Kustantaja: MDPI AG

Julkaisuvuosi: 2020

Journal: Life

Volyymi: 10

Julkaisunumero: 11

Sivujen määrä: 18

eISSN: 2075-1729

DOI: http://dx.doi.org/10.3390/life10110297

Verkko-osoite: https://www.mdpi.com/2075-1729/10/11/297/htm

Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/51157224


Tiivistelmä

Nostoc (Anabaena) sp. PCC 7120 is a filamentous cyanobacterial species that fixes N2
to nitrogenous compounds using specialised heterocyst cells. Changes in
the intracellular ratio of carbon to nitrogen (C/N balance) is known to
trigger major transcriptional reprogramming of the cell, including
initiating the differentiation of vegetative cells to heterocysts.
Substantial transcriptional analysis has been performed on Nostoc
sp. PCC 7120 during N stepdown (low to high C/N), but not during C
stepdown (high to low C/N). In the current study, we shifted the
metabolic balance of Nostoc sp. PCC 7120 cultures grown at 3% CO2 by introducing them to atmospheric conditions containing 0.04% CO2
for 1 h, after which the changes in gene expression were measured using
RNAseq transcriptomics. This analysis revealed strong upregulation of
carbon uptake, while nitrogen uptake and metabolism and early stages of
heterocyst development were downregulated in response to the shift to
low CO2. Furthermore, gene expression changes revealed a
decrease in photosynthetic electron transport and increased
photoprotection and reactive oxygen metabolism, as well a decrease in
iron uptake and metabolism. Differential gene expression was largely
attributed to change in the abundances of the metabolites
2-phosphoglycolate and 2-oxoglutarate, which signal a rapid shift from
fluent photoassimilation to glycolytic metabolism of carbon after
transition to low CO2. This work shows that the C/N balance in Nostoc
sp. PCC 7120 rapidly adjusts the metabolic strategy through
transcriptional reprogramming, enabling survival in the fluctuating
environment.


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Last updated on 2022-07-04 at 18:17