A1 Journal article – refereed

C6 peptide enzyme immunoassay in Lyme borreliosis serology




List of Authors: Rouhiainen M, Pietikäinen A, Kortela E, Kanerva MJ, Oksi J, Hytönen J

Publication year: 2021

Journal: Journal of Microbiological Methods

Journal name in source: Journal of microbiological methods

Journal acronym: J Microbiol Methods

Volume number: 180

ISSN: 0167-7012

eISSN: 1872-8359

DOI: http://dx.doi.org/10.1016/j.mimet.2020.106122


Abstract
The cut-off values used in C6 peptide-based enzyme immunoassay (EIA), a widely used test in Lyme borreliosis (LB) serology, have not been thoroughly analysed. The objective of the study was to examine the performance of the C6 EIA, and to determine optimal cut-off values for the test. The analysed data contained results of 1368 serum samples. C6 EIA index values were compared statistically with the immunoblot (IB) test results. The identified cut-off values were further tested in a well-defined LB patient cohort. Cut-off value 1.6 appeared to be optimal when C6 EIA was used as a stand-alone test. When using C6 EIA as the first-tier test, the optimal cut-off values were 0.9 and 2.4 for negative and positive results. When C6 EIA was used as a second-tier test, samples yielding C6 index values ≥3.0 could be considered positive. The identified cut-off values had also a high sensitivity to identify seropositivity among definite LB patients. The identified cut-off values refine the role of C6 EIA in LB serology. Importantly, the use of C6 EIA leads to a reduction in the number of samples that need to be analysed using an IB, thus also reducing the costs. Two alternative workflows for LB serology including the C6 EIA are suggested.

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Last updated on 2021-24-06 at 09:26