A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein
Tekijät: Peltomaa R, Fikacek S, Benito-Pena E, Barderas R, Head T, Deo S, Daunert S, Moreno-Bondi MC
Kustantaja: Springer
Julkaisuvuosi: 2020
Lehti:Microchimica Acta
Tietokannassa oleva lehden nimiMICROCHIMICA ACTA
Lehden akronyymi: Microchimica Acta
Artikkelin numero: 547
Vuosikerta: 187
Numero: 10
Sivujen määrä: 11
ISSN: 0026-3672
DOI: https://doi.org/10.1007/s00604-020-04538-7
Tiivistelmä
The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G-coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL(-1) (corresponding to 420 mu g kg(-1) in food samples) and an IC50 value of 11.0 ng mL(-1). The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD<20%, n=3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained.
The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G-coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng mL(-1) (corresponding to 420 mu g kg(-1) in food samples) and an IC50 value of 11.0 ng mL(-1). The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD<20%, n=3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained.
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