Refereed journal article or data article (A1)

Kinetic and NMR spectroscopic study of the Chemical Stability and Reaction Pathways of Sugar Nucleotides




List of Authors: Jaakkola Juho, Nieminen Anu, Kivelä Henri, Korhonen Heidi, Tähtinen Petri, Mikkola Satu

Publisher: Taylor and Francis

Publication year: 2021

Journal: Nucleosides, Nucleotides and Nucleic Acids

Volume number: 40

Issue number: 2

Number of pages: 16

ISSN: 1525-7770

eISSN: 1532-2335

DOI: http://dx.doi.org/10.1080/15257770.2020.1856870

Self-archived copy’s web address: https://research.utu.fi/converis/portal/Publication/50741560


Abstract

The alkaline cleavage of two types of sugar nucleotides has been studied by 1H and 31P NMR in order to obtain information on the stability and decomposition pathways in in aqueous solutions under alkaline conditions. The reaction of glucose 1-UDP is straightforward, and products are easy to identify. The results obtained with ribose 5-UDP and ribose 5-phosphate reveal, in contrast, a more complex reaction system than expected, and the identification of individual intermediate species was not possible. Even though definite proof for the mechanisms previously proposed could not be obtained, all the spectroscopic evidence is consistent with them. Results also emphasise the significant effect of conditions, pH, ionic strength and temperature, on the reactivity under chemical conditions.


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Downloadable publication

This is an electronic reprint of the original article.
This reprint may differ from the original in pagination and typographic detail. Please cite the original version.




Last updated on 2022-03-03 at 11:04