A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Characterization of Visceral Adipose Tissue Proteome Reveals Metabolic Changes and Inflammatory Signatures in Severe Obesity
Tekijät: Dadson, Prince; Honka, Miikka‐Juhani; Suomi, Tomi; Rokka, Anne; Kauhanen, Saila; Salminen, Paulina; Helmiö, Mika; James, Peter; Elo, Laura L.; Olkkonen, Vesa M.; Nuutila Pirjo
Kustantaja: John Wiley & Sons
Julkaisuvuosi: 2025
Lehti:: Obesity
Artikkelin numero: oby.70041
ISSN: 1930-7381
eISSN: 1930-739X
DOI: https://doi.org/10.1002/oby.70041
Verkko-osoite: https://doi.org/10.1002/oby.70041
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/504633218
Objective: Severe obesity poses a major public health concern due to its links with cardiometabolic complications and mortality. Visceral adipose tissue (VAT) plays a key role in these processes through distinct molecular features. This study aimed to characterize the VAT proteome of individuals with severe obesity and investigate its association with serum metabolic biomarkers.
Methods: A cross-sectional analysis was performed for 46 individuals with severe obesity undergoing metabolic bariatric surgery and 17 healthy controls undergoing elective abdominal surgery. VAT proteomes were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS), and serum metabolites were quantified using nuclear magnetic resonance-based metabolomics.
Results: LC-MS/MS identified 22 differentially expressed proteins (FDR < 0.05) in VAT with 12 downregulated and 10 upregulated in severe obesity. Downregulated proteins included mitochondrial enzymes involved in substrate metabolism and mitochondrial transmembrane transport. Circulating glucose, valine, and isoleucine correlated negatively with VAT mitochondrial transmembrane and electron transport proteins. Upregulated proteins were associated with inflammation, immune activation, oxidative stress, cytoskeletal remodeling, and protein turnover.
Conclusions: These findings demonstrate significant molecular alterations in the VAT proteome associated with severe obesity, providing insights into the underlying mechanisms of metabolic disease. The differentially expressed proteins may serve as biomarkers or therapeutic targets for obesity-related complications.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
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Funding: P.D. received funding from the Finnish Medical Association, Finnish Diabetes Research Foundation, Finnish-Norwegian Medical Foundation, Instrumentarium Science Foundation, Päivikki and Sakari Sohlberg Foundation, Jalmari and Rauha Ahokas Foundation, and Aarne Koskelo Foundation. M.-J.H. was supported by the Finnish Diabetes Research Foundation, the Research Council of Finland (grant 332151), and COMEGIR s.r.l. Mass spectrometry analysis was performed at the Turku Proteomics Facility, University of Turku and Åbo Akademi University. The facility is supported by Biocenter Finland. The group of V.M.O. was supported by Sigrid Jusélius Foundation, Liv och Hälsa Foundation, the Finnish Diabetes Research Foundation, Magnus Ehrnrooth Foundation, Finnish Society of Sciences and Letters and Jane and Aatos Erkko Foundation. L.L.E. reports grants from the European Research Council ERC (677943), European Union's Horizon 2020 research and innovation programme (955321), Research Council of Finland (310561, 314443, 329278, 335434, 335611 and 341342), and Sigrid Juselius Foundation during the conduct of the study. Our research is also supported by Biocenter Finland and ELIXIR Finland. This work was conducted within the Finnish Centre of Excellence in Molecular Imaging in Cardiovascular and Metabolic Research and was supported by the Research Council of Finland (grant 307402 to P.N.), the University of Turku, University Hospital, and Åbo Akademi University (Finland). The funding agencies did not participate in study design, collection, analysis, or interpretation of data, writing of the report, or the decision to submit the article for publication.
