A2 Review article in a scientific journal

Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

List of Authors: López-Labrador FX, Brown JR, Fischer N, Harvala H, Van Boheemen S, Cinek O, Sayiner A, Madsen TV, Auvinen E, Kufner V, Huber M, Rodriguez C, Jonges M, Hönemann M, Susi P, Klapper PE, Sousa H, Hernandez M, Pérez-Cataluňa A, der Hoek LV, Molenkamp R, Couto N, Schuurman R, Simmonds P, Leuzinger K, Höper D, Beer M, Feltkamp MCW, Kamminga S, Keyaerts E, Rodríguez-Díaz J, Kroes ACM, Buesa J, Nielsen XC, Puchhammer-Stöckl E, de Vries JJC, de Vries JJC, Breuer J, Claas ECJ

Publication year: 2021

Journal: Journal of Clinical Virology

Journal name in source: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

Journal acronym: J Clin Virol

Volume number: 134

Number of pages: 8

ISSN: 1386-6532

eISSN: 1873-5967

DOI: http://dx.doi.org/10.1016/j.jcv.2020.104691

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

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Last updated on 2021-24-06 at 08:10