A2 Vertaisarvioitu katsausartikkeli tieteellisessä lehdessä
Analysis of Methylated Quaternary Ammonium Compounds Using Hydrophilic Interaction Liquid Chromatography Combined With Mass Spectrometry
Tekijät: Zarei, Iman; Babu, Ambrin Farizah; Koistinen, Ville M.; Tuomainen, Marjo; Karlund, Anna; Haikonen, Retu; Lehtonen, Marko; Hanhineva, Kati
Kustantaja: Wiley
Kustannuspaikka: HOBOKEN
Julkaisuvuosi: 2025
Journal: Mass Spectrometry Reviews
Tietokannassa oleva lehden nimi: Mass Spectrometry Reviews
Lehden akronyymi: MASS SPECTROM REV
Sivujen määrä: 13
ISSN: 0277-7037
eISSN: 1098-2787
DOI: https://doi.org/10.1002/mas.21942
Verkko-osoite: https://doi.org/10.1002/mas.21942
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/498507426
Liquid chromatography-mass spectrometry (LC-MS) is a powerful technique for the detection and quantification of methylated quaternary ammonium compounds (mQACs), such as acylcarnitines and methylated amino-acid-derived (betainized) compounds, in biological matrices. Due to their high polarity and permanent charge, mQACs present analytical challenges, particularly in achieving efficient chromatographic retention and resolution. Here, we focus on the application of hydrophilic interaction liquid chromatography combined with mass spectrometric (HILIC-MS), for the analysis of these compound classes in biological samples. We highlight practical considerations in their analysis, including their MS/MS fragmentation patterns and identification in positive electrospray mode (ESI)+, to support researchers working with mQACs in targeted or untargeted metabolomics studies.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
Julkaisussa olevat rahoitustiedot:
This work was financially supported by the Horizon 2020 research and innovation programme of European Union (Grant 874739 for the LongITools project), the Research Council of Finland (nos. 321716 and 334814), and Jane and Aatos Erkko Foundation.
