A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Whole-tissue biopsy phenotyping of three-dimensional tumours reveals patterns of cancer heterogeneity
Tekijät: Tanaka N, Kanatani S, Tomer R, Sahlgren C, Kronqvist P, Kaczynska D, Louhivuori L, Kis L, Lindh C, Mitura P, Stepulak A, Corvigno S, Hartman J, Micke P, Mezheyeuski A, Strell C, Carlson JW, Moro CF, Dahlstrand H, Ostman A, Matsumoto K, Wiklund P, Oya M, Miyakawa A, Deisseroth K, Uhlen P
Kustantaja: NATURE PUBLISHING GROUP
Julkaisuvuosi: 2017
Journal: Nature Biomedical Engineering
Tietokannassa oleva lehden nimi: NATURE BIOMEDICAL ENGINEERING
Lehden akronyymi: NAT BIOMED ENG
Vuosikerta: 1
Numero: 10
Aloitussivu: 796
Lopetussivu: 806
Sivujen määrä: 11
ISSN: 2157-846X
DOI: https://doi.org/10.1038/s41551-017-0139-0
Intratumoral heterogeneity is a critical factor when diagnosing and treating patients with cancer. Marked differences in the genetic and epigenetic backgrounds of cancer cells have been revealed by advances in genome sequencing, yet little is known about the phenotypic landscape and the spatial distribution of intratumoral heterogeneity within solid tumours. Here, we show that three-dimensional light-sheet microscopy of cleared solid tumours can identify unique patterns of phenotypic heterogeneity, in the epithelial-to-mesenchymal transition and in angiogenesis, at single-cell resolution in whole formalin-fixed paraffin-embedded (FFPE) biopsy samples. We also show that cleared FFPE samples can be re-embedded in paraffin after examination for future use, and that our tumour-phenotyping pipeline can determine tumour stage and stratify patient prognosis from clinical samples with higher accuracy than current diagnostic methods, thus facilitating the design of more efficient cancer therapies.
