A1 Refereed original research article in a scientific journal

Use of Nicotiana tabacum transplastomic plants engineered to express a His-tagged CP47 for the isolation of functional photosystem II core complexes




AuthorsPagliano C, Bersanini L, Cella R, Longoni P, Pantaleoni L, Dass A, Leelavathi S, Reddy VS

PublisherELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

Publication year2017

JournalPlant Physiology and Biochemistry

Journal name in sourcePLANT PHYSIOLOGY AND BIOCHEMISTRY

Journal acronymPLANT PHYSIOL BIOCH

Volume111

First page 266

Last page273

Number of pages8

ISSN0981-9428

eISSN1873-2690

DOIhttps://doi.org/10.1016/j.plaphy.2016.12.009


Abstract
This work focuses on the development of a molecular tool for purification of Photosystem II (MI) from Nicotiana tabacum (L). To this end, the chloroplast psbB gene encoding the CP47 PSII subunit was replaced with an engineered version of the same gene containing a C-terminal His-tag. Molecular analyses assessed the effective integration of the recombinant gene and its expression. Despite not exhibiting any obvious phenotype, the transplastomic plants remained heteroplasmic even after three rounds of regeneration under antibiotic selection. However, the recombinant His-tagged CP47 protein associated in vivo to the other PSII subunits allowing the isolation of a functional PSII core complex, although with low yield of extraction. These results will open up possible perspectives for further spectroscopic and structural studies. (C) 2016 Elsevier Masson SAS. All rights reserved.



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