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Profiling the antidiabetic potential of GC-MS compounds identified from the methanolic extract of Spilanthes filicaulis: experimental and computational insight




TekijätOjo, Oluwafemi Adeleke; Ogunlakin, Akingbolabo Daniel; Gyebi, Gideon Ampoma; Ayokunle, Damilare IyinKristi; Odugbemi, Adeshina Isaiah; Babatunde, Dare Ezekiel; Akintunde, Emmanuel Adewuni; Ezea, Samson Chukwuemeka; Asogwa, Nnaemeka Tobechukwu; Asaleye, Rotdelmwa Maimako; Ojo, Adebola Busola

KustantajaTAYLOR & FRANCIS INC

KustannuspaikkaPHILADELPHIA

Julkaisuvuosi2025

JournalJournal of Biomolecular Structure and Dynamics

Tietokannassa oleva lehden nimiJOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

Lehden akronyymiJ BIOMOL STRUCT DYN

Vuosikerta43

Numero3

Aloitussivu1392

Lopetussivu1413

Sivujen määrä22

ISSN0739-1102

eISSN1538-0254

DOIhttps://doi.org/10.1080/07391102.2023.2291828


Tiivistelmä
This study examines the nutritional composition, phytochemical profiling, and antioxidant, antidiabetic, and anti-inflammatory potential of a methanolic extract of Spilanthes filicaulis leaves (MESFL) via in vitro, ex vivo, and in silico studies. In vitro antioxidant, antidiabetic, and anti-inflammatory activities were examined. In the ex vivo study, liver tissues were subjected to FeSO4-induced oxidative damage and treated with varying concentrations of MESFL. MESFL contains a reasonable amount of nitrogen-free extract, moisture, ash content, crude protein, and fat, with a lesser amount of crude fiber. According to GC-MS analysis, MESFL contains ten compounds, the most abundant of which are 13-octadecenal and Ar-tumerone. In this study, MESFL demonstrated anti-inflammatory activities via membrane stabilizing properties, proteinase inhibition, and inhibition of protein denaturation (IC50 = 72.75 +/- 11.06 mu g/mL). MESFL also strongly inhibited both alpha-amylase (IC50 = 307.02 +/- 4.25 mu g/mL) and alpha-glucosidase (IC50 = 215.51 +/- 0.47 mu g/mL) activities. Our findings also showed that FeSO4-induced tissue damage decreased the levels of GSH, SOD, and CAT activities while increasing the levels of MDA. In contrast, treatment with MESFL helped to restore these parameters to near-normal levels, which signifies that MESFL has great potential to address complications from oxidative stress. Furthermore, the in silico interaction of the GCMS-identified phytochemicals with the active sites of alpha-amylase and alpha-glucosidase via molecular and ensembled-based docking displayed strong binding affinities of Ar-tumerone and 4-hydroxy-3-methylacetophenone to alpha-amylase and alpha-glucosidase, respectively. Taken together, the biological activities of MESFL might be a result of the effects of these secondary metabolites.Communicated by Ramaswamy H. Sarma



Last updated on 2025-25-06 at 13:56