A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

Enhanced antibody responses in CD19-Cre mice




TekijätCunha, Diogo M.; Hernández-Pérez, Sara; Mattila, Pieta K.

KustantajaSpringer Nature

Julkaisuvuosi2025

JournalScientific Reports

Tietokannassa oleva lehden nimiScientific Reports

Artikkelin numero1348

Vuosikerta15

eISSN2045-2322

DOIhttps://doi.org/10.1038/s41598-024-83954-8

Verkko-osoitehttps://doi.org/10.1038/s41598-024-83954-8

Rinnakkaistallenteen osoitehttps://research.utu.fi/converis/portal/detail/Publication/485023896


Tiivistelmä

CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.


Ladattava julkaisu

This is an electronic reprint of the original article.
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Julkaisussa olevat rahoitustiedot
This work was supported by the Research Council of Finland’s Flagship InFLAMES (decision numbers: 337530 and 357910) and project funding (decision numbers: 296684, 327378, and 339810 to P.K.M.), and the Sigrid Jusélius (to P.K.M.) and Finnish Cultural Foundations (D.M.C. and S.H-P).


Last updated on 2025-23-06 at 14:37