A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Enhanced antibody responses in CD19-Cre mice
Tekijät: Cunha, Diogo M.; Hernández-Pérez, Sara; Mattila, Pieta K.
Kustantaja: Springer Nature
Julkaisuvuosi: 2025
Journal: Scientific Reports
Tietokannassa oleva lehden nimi: Scientific Reports
Artikkelin numero: 1348
Vuosikerta: 15
eISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-024-83954-8
Verkko-osoite: https://doi.org/10.1038/s41598-024-83954-8
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/485023896
CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.
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This work was supported by the Research Council of Finland’s Flagship InFLAMES (decision numbers: 337530 and 357910) and project funding (decision numbers: 296684, 327378, and 339810 to P.K.M.), and the Sigrid Jusélius (to P.K.M.) and Finnish Cultural Foundations (D.M.C. and S.H-P).