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Abscisic Acid Inhibits Type 2C Protein Phosphatases via the PYR/PYL Family of START Proteins
Tekijät: Park, Sang-Youl; Fung, Pauline; Nishimura, Noriyuki; Jensen, Davin R.; Fujii, Hiroaki; Zhao, Yang; Lumba, Shelley; Santiago, Julia; Rodrigues, Americo; Chow, Tsz-Fung F.; Alfred, Simon E.; Bonetta, Dario; Finkelstein, Ruth; Provart, Nicholas J.; Desveaux, Darrell; Rodriguez, Pedro L.; McCourt, Peter; Zhu, Jian-Kang; Schroeder, Julian I.; Volkman, Brian F.; Cutler, Sean R.
Kustantaja: AMER ASSOC ADVANCEMENT SCIENCE
Kustannuspaikka: WASHINGTON
Julkaisuvuosi: 2009
Journal: Science
Tietokannassa oleva lehden nimi: SCIENCE
Lehden akronyymi: SCIENCE
Vuosikerta: 324
Numero: 5930
Aloitussivu: 1068
Lopetussivu: 1071
Sivujen määrä: 4
ISSN: 0036-8075
eISSN: 1095-9203
DOI: https://doi.org/10.1126/science.1173041
Type 2C protein phosphatases (PP2Cs) are vitally involved in abscisic acid (ABA) signaling. Here, we show that a synthetic growth inhibitor called pyrabactin functions as a selective ABA agonist. Pyrabactin acts through PYRABACTIN RESISTANCE 1 (PYR1), the founding member of a family of START proteins called PYR/PYLs, which are necessary for both pyrabactin and ABA signaling in vivo. We show that ABA binds to PYR1, which in turn binds to and inhibits PP2Cs. We conclude that PYR/PYLs are ABA receptors functioning at the apex of a negative regulatory pathway that controls ABA signaling by inhibiting PP2Cs. Our results illustrate the power of the chemical genetic approach for sidestepping genetic redundancy.
