A1 Refereed original research article in a scientific journal
Analysis of oxysterols and vitamin D metabolites in mouse brain and cell line samples by ultra-high-performance liquid chromatography-atmospheric pressure photoionization-mass spectrometry
Authors: Ahonen L, Maire FBR, Savolainen M, Kopra J, Vreeken RJ, Hankemeier T, Myohanen T, Kylli P, Kostiainen R
Publisher: ELSEVIER SCIENCE BV
Publication year: 2014
Journal: Journal of Chromatography A
Journal name in source: JOURNAL OF CHROMATOGRAPHY A
Journal acronym: J CHROMATOGR A
Volume: 1364
First page : 214
Last page: 222
Number of pages: 9
ISSN: 0021-9673
DOI: https://doi.org/10.1016/j.chroma.2014.08.088
Abstract
We have developed an ultra-high-performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometric (UHPLC-APPI-MS/MS) method for the simultaneous quantitative analyses of several oxysterols and vitamin D metabolites in mouse brain and cell line samples. An UHPLC-APPI-high resolution mass spectrometric (UHPLC-APPI-HRMS) method that uses a quadrupole-time of flight mass spectrometer was also developed for confirmatory analysis and for the identification of non-targeted oxysterols. Both methods showed good quantitative performance. Furthermore, APPI provides high ionization efficiency for determining oxysterols and vitamin D related compounds without the time consuming derivatization step needed in the conventionally used electrospray ionization method to achieve acceptable sensitivity. Several oxysterols were quantified in mouse brain and cell line samples. Additionally, 25-hydroxyvitamin D-3 was detected in mouse brain samples for the first time. (C) 2014 Elsevier B.V. All rights reserved.
We have developed an ultra-high-performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometric (UHPLC-APPI-MS/MS) method for the simultaneous quantitative analyses of several oxysterols and vitamin D metabolites in mouse brain and cell line samples. An UHPLC-APPI-high resolution mass spectrometric (UHPLC-APPI-HRMS) method that uses a quadrupole-time of flight mass spectrometer was also developed for confirmatory analysis and for the identification of non-targeted oxysterols. Both methods showed good quantitative performance. Furthermore, APPI provides high ionization efficiency for determining oxysterols and vitamin D related compounds without the time consuming derivatization step needed in the conventionally used electrospray ionization method to achieve acceptable sensitivity. Several oxysterols were quantified in mouse brain and cell line samples. Additionally, 25-hydroxyvitamin D-3 was detected in mouse brain samples for the first time. (C) 2014 Elsevier B.V. All rights reserved.
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