A1 Refereed original research article in a scientific journal
Enhanced new bone formation in canine maxilla by a graft of electrically polarized beta-tricalcium phosphate
Authors: Nohara K, Itoh S, Akizuki T, Nakamura M, Fukuba S, Matsuura T, Okada M, Izumi Y, Iwata T, Yamashita K
Publisher: WILEY
Publication year: 2020
Journal: Journal of Biomedical Materials Research Part B: Applied Biomaterials
Journal name in source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
Journal acronym: J BIOMED MATER RES B
Volume: 108
Issue: 7
First page : 2820
Last page: 2826
Number of pages: 7
ISSN: 1552-4973
eISSN: 1552-4981
DOI: https://doi.org/10.1002/jbm.b.34612(external)
Abstract
We succeeded in the electrical polarization of beta-tricalcium phosphate (beta-TCP) granules and performed an unprecedented attempt to implant them into maxillary bone defects in canines to confirm their ability to facilitate new bone formation. Two holes were drilled into each maxilla half of a canine and filled with electrically polarized and nonpolarized beta-TCP granules (grouping assignment was decided randomly). The implanted specimens were dissected en bloc and used for microcomputed tomography (mu CT) observations and histological analyses 4 and 8 weeks after the operation. New bone ingrowth in the bone hole progressed over time from the superficial layer of the cortex toward the inner cancellous bone. The percentage area of new bone in the bone hole, as measured by mu CT in the sagittal plane, was significantly larger after 4 and 8 weeks, and that measured by H&E-stained specimens in the transverse plane after 4 weeks was significantly larger in the polarized group than in the nonpolarized group. In addition to the structural stability and chemical characteristics of the beta-TCP granules, electrical stimulation bears influence not indirectly but directly on osteogenic and vessel cells, which might work cooperatively for the early initiation of the bone formation process.
We succeeded in the electrical polarization of beta-tricalcium phosphate (beta-TCP) granules and performed an unprecedented attempt to implant them into maxillary bone defects in canines to confirm their ability to facilitate new bone formation. Two holes were drilled into each maxilla half of a canine and filled with electrically polarized and nonpolarized beta-TCP granules (grouping assignment was decided randomly). The implanted specimens were dissected en bloc and used for microcomputed tomography (mu CT) observations and histological analyses 4 and 8 weeks after the operation. New bone ingrowth in the bone hole progressed over time from the superficial layer of the cortex toward the inner cancellous bone. The percentage area of new bone in the bone hole, as measured by mu CT in the sagittal plane, was significantly larger after 4 and 8 weeks, and that measured by H&E-stained specimens in the transverse plane after 4 weeks was significantly larger in the polarized group than in the nonpolarized group. In addition to the structural stability and chemical characteristics of the beta-TCP granules, electrical stimulation bears influence not indirectly but directly on osteogenic and vessel cells, which might work cooperatively for the early initiation of the bone formation process.
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