A1 Refereed original research article in a scientific journal
Ga-68-DOTAVAP-P1 PET imaging capable of demonstrating the phase of inflammation in healing bones and the progress of infection in osteomyelitic bones
Authors: Lankinen P, Maekinen TJ, Poeyhoenen TA, Virsu P, Salomaeki S, Hakanen AJ, Jalkanen S, Aro HT, Roivainen A
Publisher: SPRINGER
Publication year: 2008
Journal: European Journal of Nuclear Medicine and Molecular Imaging
Journal name in source: EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
Journal acronym: EUR J NUCL MED MOL I
Volume: 35
Issue: 2
First page : 352
Last page: 364
Number of pages: 13
ISSN: 1619-7070
DOI: https://doi.org/10.1007/s00259-007-0637-5
Abstract
Purpose Differentiation between bacterial infection and nonbacterial inflammation remains a diagnostic challenge. Vascular adhesion protein 1 (VAP-1) is a human endothelial protein whose cell surface expression is induced under inflammatory conditions, thus making it a highly promising target molecule for studying inflammatory processes in vivo. We hypothesized that positron emission tomography (PET) with gallium-68-labeled 1,4,7,10-tetraazacyclododecane-N',N '',N''',N ''''-tetraacetic acid-peptide targeted to VAP-1 (Ga-68-DOTAVAP-P1) could be feasible for imaging the early inflammatory and infectious processes in healing bones.Materials and methods Thirty-four Sprague-Dawley rats with diffuse Staphylococcus aureus tibial osteomyelitis and 34 rats with healing cortical bone defects (representing the inflammation stage of healing) were PET imaged using Ga-68-DOTAVAP-P1 as a tracer. In addition, peripheral quantitative computed tomography and conventional radiography were performed. Bone samples for quantitative bacteriology and specimens were also processed for histomorphometry of inflammatory and infectious reactions.Results PET imaging showed an uptake of Ga-68-DOTAVAP-P1 in both the osteomyelitic bones and the healing cortical bone defects during the first 36 h after surgery. Thereafter, only the osteomyelitic tibias were delineated by PET. The osteomyelitic and control animals showed a similar uptake of the Ga-68-DOTAVAP-P1 at 24 h, whereas a significant difference was observed at 7 days (p < 0.0001).Conclusions The current study showed that PET imaging with the new Ga-68-DOTAVAP-P1 is capable of accurately demonstrating the phase of inflammation in healing bones and the progress of bacterial infection in osteomyelitic bones. Consequently, this novel imaging agent allowed for the differentiation of bone infection due to S. aureus and normal bone healing as soon as 7 days after onset.
Purpose Differentiation between bacterial infection and nonbacterial inflammation remains a diagnostic challenge. Vascular adhesion protein 1 (VAP-1) is a human endothelial protein whose cell surface expression is induced under inflammatory conditions, thus making it a highly promising target molecule for studying inflammatory processes in vivo. We hypothesized that positron emission tomography (PET) with gallium-68-labeled 1,4,7,10-tetraazacyclododecane-N',N '',N''',N ''''-tetraacetic acid-peptide targeted to VAP-1 (Ga-68-DOTAVAP-P1) could be feasible for imaging the early inflammatory and infectious processes in healing bones.Materials and methods Thirty-four Sprague-Dawley rats with diffuse Staphylococcus aureus tibial osteomyelitis and 34 rats with healing cortical bone defects (representing the inflammation stage of healing) were PET imaged using Ga-68-DOTAVAP-P1 as a tracer. In addition, peripheral quantitative computed tomography and conventional radiography were performed. Bone samples for quantitative bacteriology and specimens were also processed for histomorphometry of inflammatory and infectious reactions.Results PET imaging showed an uptake of Ga-68-DOTAVAP-P1 in both the osteomyelitic bones and the healing cortical bone defects during the first 36 h after surgery. Thereafter, only the osteomyelitic tibias were delineated by PET. The osteomyelitic and control animals showed a similar uptake of the Ga-68-DOTAVAP-P1 at 24 h, whereas a significant difference was observed at 7 days (p < 0.0001).Conclusions The current study showed that PET imaging with the new Ga-68-DOTAVAP-P1 is capable of accurately demonstrating the phase of inflammation in healing bones and the progress of bacterial infection in osteomyelitic bones. Consequently, this novel imaging agent allowed for the differentiation of bone infection due to S. aureus and normal bone healing as soon as 7 days after onset.
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