A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Single-cell transcriptome analysis of the early immune response in the lymph nodes of Borrelia burgdorferi-infected mice
Tekijät: Rinne, Varpu; Gröndahl-Yli-Hannuksela, Kirsi; Fair-Mäkelä, Ruth; Salmi, Marko; Rantakari, Pia; Lönnberg, Tapio; Alinikula, Jukka; Pietikäinen, Annukka; Hytönen, Jukka
Kustantaja: Elsevier
Julkaisuvuosi: 2024
Journal: Microbes and Infection
Tietokannassa oleva lehden nimi: Microbes and infection
Lehden akronyymi: Microbes Infect
ISSN: 1286-4579
eISSN: 1769-714X
DOI: https://doi.org/10.1016/j.micinf.2024.105424
Verkko-osoite: https://doi.org/10.1016/j.micinf.2024.105424
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/458900471
Lyme borreliosis is a disease caused by Borrelia burgdorferi sensu lato bacteria. Borrelia burgdorferi is known to induce prolonged extrafollicular immune responses and abnormal germinal centre formation. The infection fails to generate a neutralizing type of immunity, eventually establishing a persistent infection. Here, we performed single-cell RNA sequencing to characterize the immune landscape of lymph node lymphocytes during the early Borrelia burgdorferi infection in a murine model. Our results indicate key features of an extrafollicular immune response four days after Borrelia burgdorferi infection, including notable B cell proliferation, immunoglobulin class switching to IgG3 and IgG2b isotypes, plasmablast differentiation, and the presence of extrafollicular B cells identified through immunohistochemistry. Additionally, we found infection-derived upregulation of suppressor of cytokine signalling genes Socs1 and Socs3, along with downregulation of genes associated with MHC II antigen presentation in B cells. Our results support the central role of B cells in the immune response of a Borrelia burgdorferi infection, and provide cues of mechanisms behind the determination between extrafollicular and germinal centre responses during Borrelia burgdorferi infection.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
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This work was supported by the Special Governmental Fund for University Hospitals, and the Sakari Alhopuro foundation.
