A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Hsp70s transcription-translation relationship depends on the heat shock temperature in zebrafish
Tekijät: Giovanna Mottola, Mikko Nikinmaa, Katja Anttila
Kustantaja: Elsevier Inc.
Julkaisuvuosi: 2020
Journal: Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology
Tietokannassa oleva lehden nimi: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
Lehden akronyymi: Comp Biochem Physiol A Mol Integr Physiol
Artikkelin numero: 110629
Vuosikerta: 240
Sivujen määrä: 6
ISSN: 1095-6433
eISSN: 1531-4332
DOI: https://doi.org/10.1016/j.cbpa.2019.110629
Tiivistelmä
Virtually all organisms respond to heat shock by transcription of genes encoding for heat shock proteins (HSPs), but the mechanisms behind post-transcriptional regulation are not known in detail. When we exposed zebrafish to 5 and 7 °C above normal rearing temperature for 30 min, hsp70 mRNA expression was 28 and 150 -fold higher than in control, respectively. Protein expression, on the other hand, showed no significant change at the +5 °C and a 2-fold increase at the +7 °C exposure. This suggests that the transcription of hsp70 gene does not immediately correspond to translation to related proteins under certain stress temperatures, but, when the temperature is higher, and potentially detrimental, transcription and translation are intimately coupled. Those results confirm that temperature is an important abiotic factor involved in heat shock post-transcriptional regulation mechanisms in fish. However, further studies are needed to determine the relationship between this environmental factor and post-transcriptional regulation mechanisms. Earlier, the coupling/uncoupling of hsp transcription and translation has only been studied using cold-water fish, or zebrafish embryos. With current findings, we suggest this mechanism might be present even in adult warm water fish like the zebrafish.
Virtually all organisms respond to heat shock by transcription of genes encoding for heat shock proteins (HSPs), but the mechanisms behind post-transcriptional regulation are not known in detail. When we exposed zebrafish to 5 and 7 °C above normal rearing temperature for 30 min, hsp70 mRNA expression was 28 and 150 -fold higher than in control, respectively. Protein expression, on the other hand, showed no significant change at the +5 °C and a 2-fold increase at the +7 °C exposure. This suggests that the transcription of hsp70 gene does not immediately correspond to translation to related proteins under certain stress temperatures, but, when the temperature is higher, and potentially detrimental, transcription and translation are intimately coupled. Those results confirm that temperature is an important abiotic factor involved in heat shock post-transcriptional regulation mechanisms in fish. However, further studies are needed to determine the relationship between this environmental factor and post-transcriptional regulation mechanisms. Earlier, the coupling/uncoupling of hsp transcription and translation has only been studied using cold-water fish, or zebrafish embryos. With current findings, we suggest this mechanism might be present even in adult warm water fish like the zebrafish.
Turun yliopiston Tutkimustietojärjestelmän portaali