Interferon-gamma and interleukin-12 production in relation to gene polymorphisms in bacillus Calmette-Guerin osteitis

: Matti Korppi, Johanna Teräsjärvi, Eero Lauhkonen, Laura Pöyhönen, Heini Huhtala, Kirsi Nuolivirta, Qiushui He

Publisher: WILEY

: 2019

: Pediatrics International

: PEDIATRICS INTERNATIONAL

: PEDIATR INT

: 61

: 10

: 982

: 987

: 6

: 1328-8067

: 1442-200X

DOI: https://doi.org/10.1111/ped.13998(external)

Background Interferon-gamma (IFN-gamma) and interleukin-12 (IL-12) play a crucial role in the defense against mycobacteria, and in the response to bacillus Calmette-Guerin (BCG) vaccination. We have previously reported clinical and outcome data of 222 BCG osteitis cases diagnosed in 1960-1988 in Finland. The immunological and genetic reports have been based on 132 blood samples obtained in 2007-2008. Methods We compared IFN gamma and , IL12A and , and IL12B single-nucleotide polymorphisms (SNP) between 132 BCG osteitis patients and 99 population-based controls. In addition, stimulated production of IFN-gamma and IL-12 in cell culture was evaluated in relation to the presence of IFN gamma and IL12 wild versus variant genotypes, respectively. Results The distributions of IFN gamma , IFN gamma , IL12A , IL12A and IL12B SNP did not differ between BCG osteitis patients and Finnish population-based controls. For IFN gamma , IFN gamma and IL12A , the negative result was confirmed by comparing the minor allele frequencies (MAF) in BCG osteitis cases with those in the publicly available genome aggregation database, including data for 3,472 Finnish persons. Instead, for IL12A and IL12B , the comparison of MAF in BCG osteitis cases with those in population-based and in aggregation-based controls gave conflicting results. The presence of the wild versus variant genotype had no significant association with IL-12 or IFN-gamma production in BCG-stimulated cell cultures. Conclusion IFN gamma gene polymorphisms did not show any association with BCG osteitis after newborn vaccination.