Comparison of phenotypic and genotypic diagnosis of acute human bocavirus 1 infection in children

: Nicola Isabelle Kols, Heli Aatola, Ville Peltola, Man Xu, Zaiga Nora-Krukle, Klaus Hedman, Aurelija Zvirbliene, Hanna Toivola, Tytti Vuorinen, Juha M.Koskinen, Andrea H.L. Bruning, Andreas Christensen, Maria Söderlund-Venermo, Janne O.Koskinen

Publisher: ELSEVIER

: 2019

: Journal of Clinical Virology

: JOURNAL OF CLINICAL VIROLOGY

: J CLIN VIROL

: 120

: 17

: 19

: 3

: 1386-6532

DOI: https://doi.org/10.1016/j.jcv.2019.09.003

Background: Diagnosis of human bocavirus 1 (HBoV1) has been based on qualitative PCRs detecting HBoV1 DNA or detection of HBoV1 mRNA.
Objective: This study aims to assess whether a rapid and automated HBoV1 antigen test is suitable for diagnosis of acute HBoV1 infection.
Study design: HBoV1 antigen detection has been compared with quantitative HBoV1 DNA PCR and HBoV1 mRNA RT-PCR.

Results and conclusion: We conclude that HBoV1 antigen detection has higher clinical specificity and positive predictive value than HBoV1 DNA qualitative PCRs, yet a lower sensitivity than HBoV1 mRNA detection. Additionally, HBoV1 antigen detection is beneficial in its rapidity and availability as a point-of-care test.