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Intravitreal Pharmacokinetics in Mice: SPECT/CT Imaging and Scaling to Rabbits and Humans
Tekijät: Schmitt M, Hippeläinen E, Ravina M, Arango-Gonzalez B, Antopolsky M, Vellonen KS, Airaksinen AJ, Urtti A
Kustantaja: AMER CHEMICAL SOC
Julkaisuvuosi: 2019
Journal: Molecular Pharmaceutics
Tietokannassa oleva lehden nimi: MOLECULAR PHARMACEUTICS
Lehden akronyymi: MOL PHARMACEUT
Vuosikerta: 16
Numero: 10
Aloitussivu: 4399
Lopetussivu: 4404
Sivujen määrä: 6
ISSN: 1543-8384
eISSN: 1543-8392
DOI: https://doi.org/10.1021/acs.molpharmaceut.9b00679
Tiivistelmä
Preclinical in vivo tests of retinal drug responses are carried out in mice and rats, often after intravitreal injections. However, quantitative pharmacokinetics in the mouse eye is poorly understood. Ocular pharmacokinetics studies are usually done in rabbits. We investigated elimination of three compounds ([Tc-99m]Tc-pentetate, [In-111]In-pentetreotide, [Tc-99m]Tc-human serum albumin with molecular weights of 510.2 Da, 1506.4 Da, and 66.5 kDa, respectively) from mouse vitreous using imaging with single photon emission computed tomography/computed tomography (SPECT/CT). Increasing molecular weight decreased elimination of the compounds from the mouse eyes. Half-lives of [Tc-99m]Tc-pentetate, [In-111]In-pentetreotide, and [Tc-99m]Tc-human serum albumin in the mouse eyes were 1.8 +/- 0.5 h, 4.3 +/- 1.7 h, and 30.0 +/- 9.0 h, respectively. These values are 3-12-fold shorter than half-lives of similar compounds in the rabbit vitreous. Dose scaling factors were calculated for mouse-to-rabbit and mouse-to-man translation. They were 27-90 and 38-126, respectively, for intravitreal injections in rabbit and man. We show ocular pharmacokinetic parameters for mice and interspecies scaling factors that may augment ocular drug discovery and development.
Preclinical in vivo tests of retinal drug responses are carried out in mice and rats, often after intravitreal injections. However, quantitative pharmacokinetics in the mouse eye is poorly understood. Ocular pharmacokinetics studies are usually done in rabbits. We investigated elimination of three compounds ([Tc-99m]Tc-pentetate, [In-111]In-pentetreotide, [Tc-99m]Tc-human serum albumin with molecular weights of 510.2 Da, 1506.4 Da, and 66.5 kDa, respectively) from mouse vitreous using imaging with single photon emission computed tomography/computed tomography (SPECT/CT). Increasing molecular weight decreased elimination of the compounds from the mouse eyes. Half-lives of [Tc-99m]Tc-pentetate, [In-111]In-pentetreotide, and [Tc-99m]Tc-human serum albumin in the mouse eyes were 1.8 +/- 0.5 h, 4.3 +/- 1.7 h, and 30.0 +/- 9.0 h, respectively. These values are 3-12-fold shorter than half-lives of similar compounds in the rabbit vitreous. Dose scaling factors were calculated for mouse-to-rabbit and mouse-to-man translation. They were 27-90 and 38-126, respectively, for intravitreal injections in rabbit and man. We show ocular pharmacokinetic parameters for mice and interspecies scaling factors that may augment ocular drug discovery and development.
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