A1 Refereed original research article in a scientific journal
Radiolabeled Molecular Imaging Probes for the In Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications
Authors: Imlimthan S, Otaru S, Keinanen O, Correia A, Lintinen K, Santos HA, Airaksinen AJ, Kostiainen MA, Sarparanta M
Publisher: AMER CHEMICAL SOC
Publication year: 2019
Journal: Biomacromolecules
Journal name in source: BIOMACROMOLECULES
Journal acronym: BIOMACROMOLECULES
Volume: 20
Issue: 2
First page : 674
Last page: 683
Number of pages: 10
ISSN: 1525-7797
eISSN: 1526-4602
DOI: https://doi.org/10.1021/acs.biomac.8b01313
Abstract
Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors; however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site -specific hydrazone linkage to the terminal aldehyde of the CNC and nonsite-specific activation using 1,1'-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good bio-compatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of In-111 -labeled DOTA-CNC (and.) and In-111-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with noninvasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.
Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors; however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site -specific hydrazone linkage to the terminal aldehyde of the CNC and nonsite-specific activation using 1,1'-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good bio-compatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of In-111 -labeled DOTA-CNC (and.) and In-111-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with noninvasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.
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