The results of the examination of further spider material deposited in the Agricultural Zoology Museum of Iran, Iranian Research Institute of Plant Protection (Tehran, Iran), are reported, most of them from cereal fields and fruit orchards. A total of 634 specimens were studied, out of which, 106 species belonging to 70 genera and 27 families were identified. Five species are recorded for the fauna of Iran for the first time and documented by photos: Brigittea civica (Lucas, 1850) (Dictynidae), Pardosa roscai (Roewer, 1951) (Lycosidae), Tetragnatha isidis (Simon, 1880) (Tetragnathidae), Trachyzelotes miniglossus Levy, 2009 and Zelotes tenuis (L. Koch, 1866) (both Gnaphosidae). New provincial records are provided for additional 64 species. Earlier records of Heliophaneus aeneus (Hahn, 1832) in Iran are corrected to Heliophanus flavipes (Hahn, 1832) based on the re-examination of original material. Subsequently, H. aeneus has to be removed from the Iranian Checklist.