A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Ready to use dry-reagent PCR assays for the four common bacterial pathogens using switchable lanthanide luminescence probe system




Julkaisun tekijät: A. Lehmusvuori, M. Soikkeli, E. Tuunainen, T. Seppä, A. Spangar, K. Rantakokko-Jalava, P. von Lode, U. Karhunen, T. Soukka, S. Wittfooth

Kustantaja: ELSEVIER SCIENCE BV

Julkaisuvuosi: 2015

Journal: Journal of Microbiological Methods

Tietokannassa oleva lehden nimi: JOURNAL OF MICROBIOLOGICAL METHODS

Lehden akronyymi: J MICROBIOL METH

Volyymi: 118

Sivujen määrä: 6

ISSN: 0167-7012

DOI: http://dx.doi.org/10.1016/j.mimet.2015.08.022


Tiivistelmä

Ready to use dry-reagent PCR assays for Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas spp. and for broad-range bacteria detection were developed. The assays were based on novel switchable lanthanide probes that provide sensitive target DNA detection with exceptionally high signal-to-background ratio, thus enabling clear discrimination between positive and negative results. For example, sensitivity of three S. aureus and two S. pneumonia bacteria (colony forming units) per PCR assay was measured with fluorescence signal more than 30 times over the background signal level. The rapid and easy-to-use assays are suitable for routine clinical diagnostics without molecular biology expertise and facilities. (C) 2015 Elsevier B.V. All rights reserved.



Last updated on 2021-24-06 at 09:08