Vertaisarvioitu alkuperäisartikkeli tai data-artikkeli tieteellisessä aikakauslehdessä (A1)

In silico characterization of a calcium-dependent protein kinase gene from Hevea brasiliensis reveals prospective features for conferring multiple stress tolerance.

Julkaisun tekijätSuni Anie Mathew, R.Supriya, Thulaseedharan A

KustantajaRubber Science





Lopetussivun numero158



Identifying potential genes imparting stress tolerance is an important step for developing rubber clones that can survive in stress-prone geographical locations. This will help in increasing rubber production by extending the area under Hevea plantations. Calcium-dependent protein kinase (cdpk) is one such gene involved in multiple stress signaling pathways. In the present study, a cdpk gene and its promoter region were isolated from the high latex-yielding Hevea clone RRII 105 and characterized. The intron pattern analysis of the genomic sequence classified the gene into Group II subfamily of CDPK proteins. The in silico analysis predicted the myristoylation site, palmitoylation sites, presence of nuclear localization signal and subcellular localization, hinting its role in signal transduction, protein-protein interactions and shuttling mechanisms during stress. The sequence analysis of the promoter region showed stress-responsive cis-elements that help in regulating gene expression. The sequence alignment and 3D modeled protein structure superposition of the isolated cdpk with Arabidopsis cdpk21 is also predicted which is useful in identifying the orthologous nature between the two proteins, contributing to their functional similarity involved in multiple stress signaling. These results suggest that the isolated Hevea cdpk gene confers features for imparting multiple abiotic stress tolerance.

Last updated on 2021-24-06 at 11:16