Genotyping of clinically relevant human adenoviruses by array-in-well hybridization assay

: Ylihärsilä M, Harju E, Arppe R, Hattara L, Hölsä J, Saviranta P, Soukka T, Waris M

Publisher: WILEY-BLACKWELL

: 2013

: Clinical Microbiology and Infection

: CLINICAL MICROBIOLOGY AND INFECTION

: CLIN MICROBIOL INFEC

: 6

: 19

: 6

: 551

: 557

: 7

: 1198-743X

DOI: https://doi.org/10.1111/j.1469-0691.2012.03926.x

A robust oligonucleotide array-in-well hybridization assay using novel up-converting phosphor reporter technology was applied for genotyping clinically relevant human adenovirus types. A total of 231 adenovirus-positive respiratory, ocular swab, stool and other specimens from 219 patients collected between April 2010 and April 2011 were included in the study. After a real-time PCR amplification targeting the adenovirus hexon gene, the array-in-well assay identified the presence of B03 (n=122; 57.5% of patients), E04 (29; 13.7%), C02 (21; 9.9%), D37 (14; 6.6%), C01 (12; 5.7%), C05 (5; 2.4%), D19 (4; 1.9%), C06 (2; 0.9%), D08 (1; 0.5%), A31 (1; 0.5%) and F41 (1; 0.5%) genotypes among the clinical sample panel. The typing result was obtained for all specimens that could be amplified (n=223; 97%), and specificity of the typing was confirmed by sequencing specimens representing each of the different genotypes. No hybridization signal was obtained in adenovirus-negative specimens or specimens with other viruses (n=30). The array-in-well hybridization assay has great potential as a rapid and multiplex platform for the typing of clinically relevant human adenovirus genotypes in different specimen types.