A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
FELASA guidelines for the refinement of methods for genotyping genetically-modified rodents
Tekijät: Bonaparte D, Cinelli P, Douni E, Herault Y, Maas A, Pakarinen P, Poutanen M, Lafuente MS, Scavizzi F
Kustantaja: SAGE PUBLICATIONS INC
Julkaisuvuosi: 2013
Journal: Laboratory Animals
Tietokannassa oleva lehden nimi: LABORATORY ANIMALS
Lehden akronyymi: LAB ANIM-UK
Numero sarjassa: 3
Vuosikerta: 47
Numero: 3
Aloitussivu: 134
Lopetussivu: 145
Sivujen määrä: 12
ISSN: 0023-6772
DOI: https://doi.org/10.1177/0023677212473918
Tiivistelmä
The use of genetically-modified (GM) animals as research models continues to grow. The completion of the mouse genome sequence, together with the high-throughput international effort to introduce mutations across the mouse genome in the embryonic stem (ES) cells (www.knockoutmouse.org) facilitates an efficient way to obtain mutated mouse strains as research models. The increasing number of available mutated mouse strains and their combinations, together with the increasing complexity in the targeting approaches used, reinforces the need for guidelines that will provide information about the mouse strains and the robust and reliable methods used for their genotyping. This information, however, should be obtained with a method causing minimal discomfort to the experimental animals. We have, therefore, compiled the present document which summarizes the currently available methods for obtaining genotype information. It provides updated guidelines concerning animal identification, DNA sampling and genotyping, and the information to be kept and distributed for any mutated rodent strain.
The use of genetically-modified (GM) animals as research models continues to grow. The completion of the mouse genome sequence, together with the high-throughput international effort to introduce mutations across the mouse genome in the embryonic stem (ES) cells (www.knockoutmouse.org) facilitates an efficient way to obtain mutated mouse strains as research models. The increasing number of available mutated mouse strains and their combinations, together with the increasing complexity in the targeting approaches used, reinforces the need for guidelines that will provide information about the mouse strains and the robust and reliable methods used for their genotyping. This information, however, should be obtained with a method causing minimal discomfort to the experimental animals. We have, therefore, compiled the present document which summarizes the currently available methods for obtaining genotype information. It provides updated guidelines concerning animal identification, DNA sampling and genotyping, and the information to be kept and distributed for any mutated rodent strain.
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