A1 Refereed original research article in a scientific journal
Solid-Supported NOTA and DOTA Chelators Useful for the Synthesis of 3 '-Radiometalated Oligonucleotides
Authors: Kiviniemi A, Makela J, Makila J, Saanijoki T, Liljenback H, Poijarvi-Virta P, Lonnberg H, Laitala-Leinonen T, Roivainen A, Virta P
Publisher: AMER CHEMICAL SOC
Publication year: 2012
Journal: Bioconjugate Chemistry
Journal name in source: BIOCONJUGATE CHEMISTRY
Journal acronym: BIOCONJUGATE CHEM
Number in series: 9
Volume: 23
Issue: 9
First page : 1981
Last page: 1988
Number of pages: 8
ISSN: 1043-1802
DOI: https://doi.org/10.1021/bc300253t
Abstract
Esterified precursors of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA; 18) and 1,4,7-triazacyclononane-1,4,7-trisacetic acid (NOTA; 17,19) ligands bearing a dimethoxytritylated hydroxyl side arm were prepared and immobilized via an ester linkage to long chain alkyl amine derivatized controlled pore glass (LCAA-CPG). Oligonucleotide chains were then assembled on the hydroxyl function and conjugates were released and deprotected by a two-step cleavage with aqueous alkali and ammonia. The 3'-DOTA and 3'-NOTA conjugated oligonucleotides were converted to Ga-68 chelates by a brief treatment with [Ga-68]Cl-3 at elevated temperature. Applicability of the conjugates for in vivo imaging with positron emission tomography (PET) was verified.
Esterified precursors of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA; 18) and 1,4,7-triazacyclononane-1,4,7-trisacetic acid (NOTA; 17,19) ligands bearing a dimethoxytritylated hydroxyl side arm were prepared and immobilized via an ester linkage to long chain alkyl amine derivatized controlled pore glass (LCAA-CPG). Oligonucleotide chains were then assembled on the hydroxyl function and conjugates were released and deprotected by a two-step cleavage with aqueous alkali and ammonia. The 3'-DOTA and 3'-NOTA conjugated oligonucleotides were converted to Ga-68 chelates by a brief treatment with [Ga-68]Cl-3 at elevated temperature. Applicability of the conjugates for in vivo imaging with positron emission tomography (PET) was verified.
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