A1 Journal article – refereed

Solid-Supported NOTA and DOTA Chelators Useful for the Synthesis of 3 '-Radiometalated Oligonucleotides




List of Authors: Kiviniemi A, Makela J, Makila J, Saanijoki T, Liljenback H, Poijarvi-Virta P, Lonnberg H, Laitala-Leinonen T, Roivainen A, Virta P

Publisher: AMER CHEMICAL SOC

Publication year: 2012

Journal: Bioconjugate Chemistry

Journal name in source: BIOCONJUGATE CHEMISTRY

Journal acronym: BIOCONJUGATE CHEM

Number in series: 9

Volume number: 23

Issue number: 9

Number of pages: 8

ISSN: 1043-1802

DOI: http://dx.doi.org/10.1021/bc300253t


Abstract
Esterified precursors of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA; 18) and 1,4,7-triazacyclononane-1,4,7-trisacetic acid (NOTA; 17,19) ligands bearing a dimethoxytritylated hydroxyl side arm were prepared and immobilized via an ester linkage to long chain alkyl amine derivatized controlled pore glass (LCAA-CPG). Oligonucleotide chains were then assembled on the hydroxyl function and conjugates were released and deprotected by a two-step cleavage with aqueous alkali and ammonia. The 3'-DOTA and 3'-NOTA conjugated oligonucleotides were converted to Ga-68 chelates by a brief treatment with [Ga-68]Cl-3 at elevated temperature. Applicability of the conjugates for in vivo imaging with positron emission tomography (PET) was verified.


Research Areas


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