A1 Refereed original research article in a scientific journal
Reactive oxygen species regulate oxygen-sensitive potassium flux in rainbow trout erythrocytes
Authors: Bogdanova AY, Nikinmaa M
Publisher: ROCKEFELLER UNIV PRESS
Publication year: 2001
Journal: Journal of General Physiology
Journal name in source: JOURNAL OF GENERAL PHYSIOLOGY
Journal acronym: J GEN PHYSIOL
Volume: 117
Issue: 2
First page : 181
Last page: 190
Number of pages: 10
ISSN: 0022-1295
DOI: https://doi.org/10.1085/jgp.117.2.181
Abstract
In the present study we have investigated if reactive oxygen species are involved in the oxygen-dependent regulation of potassium-chloride cotransport activity in trout erythrocyte membrane. An increase in the oxygen level caused an increase in chloride-sensitive potassium transport (K+-Cl- cotransport). 5 mM hydrogen peroxide caused an increase in K+-Cl- cotransport at 5% oxygen. The increase in flux could be inhibited by adding extracellular catalase in the incubation. Pretreatment of the cells with mercaptopropionyl glycine (MPG), a scavenger of reactive oxygen species showing preference for hydroxyl radicals, abolished the activation of the K+-Cl- cotransporter by increased oxygen levels. The inhibition by MPG was due to the of transporter by the sulfhydryl reagent, N-ethylmaleimide, indicating that the effect of mPG was due to the scavenging of reactive oxygen species and not to the reaction of MPG with the cotransporter. Copper ions, which catalyze the production of hydroxyl radicals in the Fenton reaction, activated K+-Cl- cotransport significantly at hypoxic conditions (1% O-2). These data suggest that hydroxyl radicals, formed from O-2 in close vicinity to the cell membrane, play an important role in the oxygen-dependent activation of the K+-Cl- cotransporter.
In the present study we have investigated if reactive oxygen species are involved in the oxygen-dependent regulation of potassium-chloride cotransport activity in trout erythrocyte membrane. An increase in the oxygen level caused an increase in chloride-sensitive potassium transport (K+-Cl- cotransport). 5 mM hydrogen peroxide caused an increase in K+-Cl- cotransport at 5% oxygen. The increase in flux could be inhibited by adding extracellular catalase in the incubation. Pretreatment of the cells with mercaptopropionyl glycine (MPG), a scavenger of reactive oxygen species showing preference for hydroxyl radicals, abolished the activation of the K+-Cl- cotransporter by increased oxygen levels. The inhibition by MPG was due to the of transporter by the sulfhydryl reagent, N-ethylmaleimide, indicating that the effect of mPG was due to the scavenging of reactive oxygen species and not to the reaction of MPG with the cotransporter. Copper ions, which catalyze the production of hydroxyl radicals in the Fenton reaction, activated K+-Cl- cotransport significantly at hypoxic conditions (1% O-2). These data suggest that hydroxyl radicals, formed from O-2 in close vicinity to the cell membrane, play an important role in the oxygen-dependent activation of the K+-Cl- cotransporter.
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