A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Structure-based design and application of an engineered glutathione transferase for the development of an optical biosensor for pesticides determination
Tekijät: Chronopoulou Evangelia G., Vlachakis Dimitrios, Papageorgiou Anastassios C., Ataya Farid S., Labrou Nikolaos E.
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2019
Journal: BBA - General Subjects
Tietokannassa oleva lehden nimi: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Lehden akronyymi: BBA-GEN SUBJECTS
Vuosikerta: 1863
Numero: 3
Aloitussivu: 565
Lopetussivu: 576
Sivujen määrä: 12
ISSN: 0304-4165
DOI: https://doi.org/10.1016/j.bbagen.2018.12.004
Tiivistelmä
In the present work, a structure-based design approach was used for the generation of a novel variant of synthetic glutathione transferase (PvGmGSTU) with higher sensitivity towards pesticides. Molecular modelling studies revealed Phe117 as a key residue that contributes to the formation of the hydrophobic binding site (H-site) and modulates the affinity of the enzyme towards xenobiotic compounds. Site-saturation mutagenesis of position Phe117 created a library of,PvGmGSTU variants with altered kinetic and binding properties. Screening of the library against twenty-five different pesticides, showed that the mutant enzyme Phe117Ile displays 3-fold higher catalytic efficiency and exhibits increased affinity towards alpha-endosulfan, compared to the wild-type enzyme. Based on these catalytic features the mutant enzyme Phe117Ile was explored for the development of an optical biosensor for alpha-endosulfan. The enzyme was entrapped in alkosixylane sol-gel system in the presence of two pH indicators (bromocresol purple and phenol red). The sensing signal was based on the inhibition of the sol-gel entrapped GST, with subsequent decrease of released [H+] by the catalytic reaction, measured by sol gel entrapped indicators. The assay response at 562 nm was linear in the range pH = 4-7. Linear calibration curves were obtained for a-endosulfan in the range of 0-30 mu M. The reproducibility of the assay response, expressed by relative standard deviation, was in the order of 4.1% (N = 28). The method was successfully applied to the determination of a-endosulfan in real water samples without sample preparation steps.
In the present work, a structure-based design approach was used for the generation of a novel variant of synthetic glutathione transferase (PvGmGSTU) with higher sensitivity towards pesticides. Molecular modelling studies revealed Phe117 as a key residue that contributes to the formation of the hydrophobic binding site (H-site) and modulates the affinity of the enzyme towards xenobiotic compounds. Site-saturation mutagenesis of position Phe117 created a library of,PvGmGSTU variants with altered kinetic and binding properties. Screening of the library against twenty-five different pesticides, showed that the mutant enzyme Phe117Ile displays 3-fold higher catalytic efficiency and exhibits increased affinity towards alpha-endosulfan, compared to the wild-type enzyme. Based on these catalytic features the mutant enzyme Phe117Ile was explored for the development of an optical biosensor for alpha-endosulfan. The enzyme was entrapped in alkosixylane sol-gel system in the presence of two pH indicators (bromocresol purple and phenol red). The sensing signal was based on the inhibition of the sol-gel entrapped GST, with subsequent decrease of released [H+] by the catalytic reaction, measured by sol gel entrapped indicators. The assay response at 562 nm was linear in the range pH = 4-7. Linear calibration curves were obtained for a-endosulfan in the range of 0-30 mu M. The reproducibility of the assay response, expressed by relative standard deviation, was in the order of 4.1% (N = 28). The method was successfully applied to the determination of a-endosulfan in real water samples without sample preparation steps.
Turun yliopiston Tutkimustietojärjestelmän portaali