A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
THE EFFECT OF THE 3'-TERMINAL MONOPHOSPHATE GROUP ON THE METAL-ION-PROMOTED HYDROLYSIS OF THE PHOSPHODIESTER BONDS OF SHORT OLIGONUCLEOTIDES
Tekijät: KUUSELA S, AZHAYEV A, GUZAEV A, LONNBERG H
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 1995
Journal: Royal Society of Chemistry. Journal: Perkin Transactions 2
Tietokannassa oleva lehden nimi: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
Lehden akronyymi: J CHEM SOC PERK T 2
Numero: 6
Aloitussivu: 1197
Lopetussivu: 1202
Sivujen määrä: 6
ISSN: 0300-9580
DOI: https://doi.org/10.1039/p29950001197
The effect of 3'-terminal monophosphate group on the metal-ion-promoted hydrolysis of the phosphodiester bonds of oligonucleotides has been studied. For this purpose, the rate constants for the hydrolysis of the following oligomers in the presence of Zn2+ and its 1,5,9-triazacyclododecane chelate, Zn2+[12]aneN(3), have been determined: (i) ApUpUp(2') and ApUpUp(3'), (ii) Up(Tp)(n)T (n = 0-4 and 7), (iii) Up(Tp)(n)Tp (n = 0-4 and 7). The results obtained are used to propose a mechanism for the Zn2+ and Zn2+ [12]aneN(3) promoted hydrolysis of polynucleotides.
