Assessment of the functional response of photoreceptors plays an important role in assessing and treating vision loss. Optoretinography (ORG) is an emerging non-invasive technique that measures the photoreceptors’ functional response to external light stimuli using optical coherence tomography (OCT) or other phase-sensitive imaging modalities. Recently a novel velocity-based ORG method was demonstrated, illustrating the feasibility of measuring photoreceptor function with clinical-grade OCT systems. Here we test this technique on diseaseaffected retinae of human subjects. The disease-affected retinae exhibited altered responses when compared to a healthy volunteer. The findings indicate promise for this novel tool to find applications in the clinic and clinical research.