Triterpene Acids in Plantago major: Identification, Quantification and Comparison of Different Extraction Methods

: Tarvainen Marko, Suomela Jukka-Pekka, Kallio Heikki, Yang Baoru

Publisher: Vieweg+Teubner Verlag/GWV Fachverlage GmbH

: 2010

: Chromatographia

: 3-4

: 71

: 3

: 279

: 284

: 6

: 0009-5893

DOI: https://doi.org/10.1365/s10337-009-1439-7

Seeds and leaves of cultivated great plantain (Plantago major L.) were extd. with conventional Soxhlet extn. using a variety of solvents and with pilot scale supercrit. fluid (CO2) extn. (SFE-CO2). Hydroxy pentacyclic triterpene acids (HPTAs), oleanolic acid and ursolic acid, were identified from the SPE purified exts. with LC-(UV)-APCI-MS and quantified with LC-UV. Dried P. major leaves contained 0.07% of oleanolic acid and 0.22% of UA. Seeds had very small amts. of HPTAs, 0.005% of OA and 0.007% of ursolic acid in the oil extd. SFE-CO2 extn. without polar modifier was found not to be a suitable technol. for the leaf extn. due to the low content of lipophilic and volatile compds. Soxhlet extn. with di-Et ether and SFE-CO2 extn. were similar in the efficiency of extg. HPTAs from the seeds.

Biochemistry, cell and molecular biology