A1 Refereed original research article in a scientific journal
Involvement of polyamine binding protein D (PotD) of Synechocystis sp. PCC 6803 in spermidine uptake and excretion
Authors: Yodsang P, Pothopongsa A, Mäenpää P, Incharoensakdi A
Publication year: 2014
Journal: Current Microbiology
Volume: 69
Issue: 4
First page : 417
Last page: 422
Number of pages: 6
ISSN: 0343-8651
DOI: https://doi.org/10.1007/s00284-014-0605-9(external)
The in vivo function of polyamine binding protein D (PotD) in Synechocystis sp. PCC 6803 for the transport of spermidine was investigated using Synechocystis mutant disrupted in potD gene. The growth rate of potD mutant was similar to that of wild-type when grown in BG11 medium. However, the mutant exhibited severely reduced growth compared to the wild-type when BG11 medium was supplemented with 0.5 mM spermidine. The mutant accumulated a higher spermidine level than the wild-type when grown in the medium with or without spermidine. Transport experiments revealed that the mutant had a reduction in both the uptake and the excretion of spermidine. Moreover, [14C]spermidine-loaded wild-type and mutant cells showed a decrease of [14C]spermidine excretion when the assay medium contained exogenous spermidine. These data suggest that PotD is involved in both the uptake and the excretion of spermidine in Synechocystis cells.