A1 Refereed original research article in a scientific journal
Kainic acid-induced status epilepticus alters GABA(A) receptor subunit mRNA and protein expression in the developing rat hippocampus
Authors: Lauren HB, Lopez-Picon FR, Korpi ER, Holopainen IE
Publisher: BLACKWELL PUBLISHING
Publication year: 2005
Journal: Journal of Neurochemistry
Journal name in source: JOURNAL OF NEUROCHEMISTRY
Journal acronym: J NEUROCHEM
Volume: 94
Issue: 5
First page : 1384
Last page: 1394
Number of pages: 11
ISSN: 0022-3042
DOI: https://doi.org/10.1111/j.1471-4159.2005.03274.x
Abstract
Kainic acid-induced status epilepticus leads to structural and functional changes in inhibitory GABA(A) receptors in the adult rat hippocampus, but whether similar changes occur in the developing rat is not known. We have used in situ hybridization to study status epilepticus-induced changes in the GABA(A)alpha 1-alpha 5, beta 1-beta 3, gamma 1 and gamma 2 subunit mRNA expression in the hippocampus of 9-day-old rats during 1 week after the treatment. Immunocytochemistry was applied to detect the alpha 1, alpha 2 and beta 3 subunit proteins in the control and treated rats. In the saline-injected control rats, the alpha 1 and alpha 4 subunit mRNA expression significantly increased between the postnatal days 9-16, whereas those of alpha 2, beta 3 and gamma 2 subunits decreased. The normal developmental changes in the expression of alpha 1, alpha 2, beta 3 and gamma 2 subunit mRNAs were altered after the treatment. The immunostainings with antibodies to alpha 1, alpha 2 and beta 3 subunits confirmed the in situ hybridization findings. No neuronal death was detected in any hippocampal subregion in the treated rats. Our results show that status epilepticus disturbs the normal developmental expression pattern of GABA(A) receptor subunit in the rat hippocampus during the sensitive postnatal period of brain development. These perturbations could result in altered functional and pharmacological properties of GABA(A) receptors.
Kainic acid-induced status epilepticus leads to structural and functional changes in inhibitory GABA(A) receptors in the adult rat hippocampus, but whether similar changes occur in the developing rat is not known. We have used in situ hybridization to study status epilepticus-induced changes in the GABA(A)alpha 1-alpha 5, beta 1-beta 3, gamma 1 and gamma 2 subunit mRNA expression in the hippocampus of 9-day-old rats during 1 week after the treatment. Immunocytochemistry was applied to detect the alpha 1, alpha 2 and beta 3 subunit proteins in the control and treated rats. In the saline-injected control rats, the alpha 1 and alpha 4 subunit mRNA expression significantly increased between the postnatal days 9-16, whereas those of alpha 2, beta 3 and gamma 2 subunits decreased. The normal developmental changes in the expression of alpha 1, alpha 2, beta 3 and gamma 2 subunit mRNAs were altered after the treatment. The immunostainings with antibodies to alpha 1, alpha 2 and beta 3 subunits confirmed the in situ hybridization findings. No neuronal death was detected in any hippocampal subregion in the treated rats. Our results show that status epilepticus disturbs the normal developmental expression pattern of GABA(A) receptor subunit in the rat hippocampus during the sensitive postnatal period of brain development. These perturbations could result in altered functional and pharmacological properties of GABA(A) receptors.
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