A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Aptamer-directed lanthanide chelate self-assembly for rapid thrombin detection
Tekijät: Päkkilä H, Blom S, Kopra K, Soukka T
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 2013
Journal: Analyst
Tietokannassa oleva lehden nimi: ANALYST
Lehden akronyymi: ANALYST
Numero sarjassa: 17
Vuosikerta: 138
Numero: 17
Aloitussivu: 5107
Lopetussivu: 5112
Sivujen määrä: 6
ISSN: 0003-2654
DOI: https://doi.org/10.1039/c3an00192j
Tiivistelmä
We report a sensitive assay method for homogeneous thrombin detection. The method is based on lanthanide chelate complementation, where the luminescent complex is split into two separate label moieties, which are intrinsically non-luminescent. A luminescent mixed chelate complex is formed only when the label moieties are brought into close proximity directed by two separate binding events of aptamers to the analyte. This results in high specificity in signal generation while time-resolved fluorescence detection eliminates the short lifetime autofluorescence, which is inherent to many homogeneous assays and limits their applicability. The developed method is also very rapid as the maximum signal is obtained in just five minutes. Lanthanide chelate complementation can be applied for the detection of other proteins when two binders recognizing separate epitopes of the analyte are available.
We report a sensitive assay method for homogeneous thrombin detection. The method is based on lanthanide chelate complementation, where the luminescent complex is split into two separate label moieties, which are intrinsically non-luminescent. A luminescent mixed chelate complex is formed only when the label moieties are brought into close proximity directed by two separate binding events of aptamers to the analyte. This results in high specificity in signal generation while time-resolved fluorescence detection eliminates the short lifetime autofluorescence, which is inherent to many homogeneous assays and limits their applicability. The developed method is also very rapid as the maximum signal is obtained in just five minutes. Lanthanide chelate complementation can be applied for the detection of other proteins when two binders recognizing separate epitopes of the analyte are available.
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