A1 Refereed original research article in a scientific journal
Nodularia spumigena extract induces upregulation of mitochondrial respiratory chain complexes in spinach (Spinacia oleracea L.)
Authors: Shunmugam S, Hinttala R, Lehtimaki N, Miettinen M, Uusimaa J, Majamaa K, Sivonen K, Aro EM, Mulo P
Publisher: SPRINGER HEIDELBERG
Publication year: 2013
Journal: Acta Physiologiae Plantarum
Journal name in source: ACTA PHYSIOLOGIAE PLANTARUM
Journal acronym: ACTA PHYSIOL PLANT
Number in series: 3
Volume: 35
Issue: 3
First page : 969
Last page: 974
Number of pages: 6
ISSN: 0137-5881
DOI: https://doi.org/10.1007/s11738-012-1119-3(external)
Abstract
Nodularin, a cyclic hepatotoxic pentapeptide produced by the nitrogen-fixing cyanobacterium Nodularia spumigena, induces oxidative stress in various organisms including higher plants and algae. We have monitored the physiological consequences of N. spumigena AV1 extract exposure on terrestrial plants, specifically focusing on the mitochondrial function of Spinacia oleracea L. Our results show that exposure of the plants to the nodularin-containing extract leads to significantly increased activity of respiratory complex I and citrate synthase, as well as increased accumulation of various subunits of respiratory enzyme complexes. Moreover, upregulation of the stress-induced alternative oxidase as well as the NAD(+)-specific isocitrate dehydrogenase and mitochondrial ascorbate peroxidase was detected in the mitochondria of plants exposed to N. spumigena AV1 extract, while no difference in the carbonylation level of the mitochondrial proteins could be detected between the control and the exposed plants.
Nodularin, a cyclic hepatotoxic pentapeptide produced by the nitrogen-fixing cyanobacterium Nodularia spumigena, induces oxidative stress in various organisms including higher plants and algae. We have monitored the physiological consequences of N. spumigena AV1 extract exposure on terrestrial plants, specifically focusing on the mitochondrial function of Spinacia oleracea L. Our results show that exposure of the plants to the nodularin-containing extract leads to significantly increased activity of respiratory complex I and citrate synthase, as well as increased accumulation of various subunits of respiratory enzyme complexes. Moreover, upregulation of the stress-induced alternative oxidase as well as the NAD(+)-specific isocitrate dehydrogenase and mitochondrial ascorbate peroxidase was detected in the mitochondria of plants exposed to N. spumigena AV1 extract, while no difference in the carbonylation level of the mitochondrial proteins could be detected between the control and the exposed plants.
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