A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Inhibition of Photosystem II by the singlet oxygen sensor compounds TEMP and TEMPD
Tekijät: Hakala-Yatkin M, Tyystjarvi E
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2011
Journal: BBA - Bioenergetics
Tietokannassa oleva lehden nimi: BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Lehden akronyymi: BBA-BIOENERGETICS
Numero sarjassa: 3
Vuosikerta: 1807
Numero: 3
Aloitussivu: 243
Lopetussivu: 250
Sivujen määrä: 8
ISSN: 0005-2728
DOI: https://doi.org/10.1016/j.bbabio.2010.11.014
Tiivistelmä
2,2,6,6-tetramethylpiperidine (TEMP) and 2,2,6,6-tetramethyl-4-piperidinone (TEMPD) have earlier been used to quantify singlet oxygen produced by plant material. Both compounds were found to cause severe side effects on Photosystem II. Addition of TEMP or TEMPD to thylakoids immediately stabilized the reduced state of the Q(A) electron acceptor and destabilized the reduced state of the Q(B) acceptor, causing decrease in the driving force of forward electron transfer. Oxygen evolution, thermoluminescence and fluorescence measurements indicated that the number of functional PSII units decreased during incubation of thylakoids with TEMP or TEMPD. Singlet oxygen determinations in photosynthetic systems with piperidine derivatives should be interpreted with care. (C) 2010 Elsevier B.V. All rights reserved.
2,2,6,6-tetramethylpiperidine (TEMP) and 2,2,6,6-tetramethyl-4-piperidinone (TEMPD) have earlier been used to quantify singlet oxygen produced by plant material. Both compounds were found to cause severe side effects on Photosystem II. Addition of TEMP or TEMPD to thylakoids immediately stabilized the reduced state of the Q(A) electron acceptor and destabilized the reduced state of the Q(B) acceptor, causing decrease in the driving force of forward electron transfer. Oxygen evolution, thermoluminescence and fluorescence measurements indicated that the number of functional PSII units decreased during incubation of thylakoids with TEMP or TEMPD. Singlet oxygen determinations in photosynthetic systems with piperidine derivatives should be interpreted with care. (C) 2010 Elsevier B.V. All rights reserved.
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