The R78K and D117E active-site variants of Saccharomyces cerevisiae soluble inorganic pyrophosphatase: Structural studies and mechanistic implications



: Tuominen V, Heikinheimo P, Kajander T, Torkkel T, Hyytia T, Kapyla J, Lahti R, Cooperman BS, Goldman A

Publisher: ACADEMIC PRESS LTD

: 1998

: JOURNAL OF MOLECULAR BIOLOGY

: J MOL BIOL

: 284

: 5

: 1565

: 1580

: 16

: 0022-2836

DOI: https://doi.org/10.1006/jmbi.1998.2266


These new structures, together with parallel functional studies measuring catalytic efficiency and ligand (metal ion, PPi and P-i) binding, provide strong evidence against a proposed mechanism in which Wat1 is considered unimportant for hydrolysis. They thus support the notion that PPase shares mechanistic similarity with the "two-metal ion" mechanism of polymerases. (C) 1998 Academic Press.



Last updated on 2024-26-11 at 22:14