A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Piecemeal microautophagy of the nucleus Genetic and morphological traits
Tekijät: Krick R, Muhe Y, Prick T, Bredschneider M, Bremer S, Wenzel D, Eskelinen EL, Thumm M
Kustantaja: LANDES BIOSCIENCE
Julkaisuvuosi: 2009
Journal: Autophagy
Tietokannassa oleva lehden nimi: AUTOPHAGY
Lehden akronyymi: AUTOPHAGY
Vuosikerta: 5
Numero: 2
Aloitussivu: 270
Lopetussivu: 272
Sivujen määrä: 3
ISSN: 1554-8627
DOI: https://doi.org/10.1091/mbc.E08-04-0363
Tiivistelmä
Nucleus-vacuole (NV) junctions are formed in Saccharomyces cerevisiae through interactions between Vac8 in the vacuole membrane and Nvj1 in the perinuclear ER. Upon starvation, vesicles containing part of the nucleus emanate from these contact sites and finally pinch off into invaginations of the vacuole. Due to its morphological similarity to microautophagy this process had been termed "piecemeal microautophagy of the nucleus" (PMN). We recently discovered that a number of ATG genes required for macroautophagy and micropexophagy are also required for PMN and accordingly named it micronucleophagy. Therefore, PMN represents a novel model system to investigate the functions of the highly conserved but poorly understood core autophagic apparatus. We here extend the morphological analysis of PMN using immunogold and freeze fracture electron microscopy.
Nucleus-vacuole (NV) junctions are formed in Saccharomyces cerevisiae through interactions between Vac8 in the vacuole membrane and Nvj1 in the perinuclear ER. Upon starvation, vesicles containing part of the nucleus emanate from these contact sites and finally pinch off into invaginations of the vacuole. Due to its morphological similarity to microautophagy this process had been termed "piecemeal microautophagy of the nucleus" (PMN). We recently discovered that a number of ATG genes required for macroautophagy and micropexophagy are also required for PMN and accordingly named it micronucleophagy. Therefore, PMN represents a novel model system to investigate the functions of the highly conserved but poorly understood core autophagic apparatus. We here extend the morphological analysis of PMN using immunogold and freeze fracture electron microscopy.
Turun yliopiston Tutkimustietojärjestelmän portaali