A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
The adrenergic volume changes of immature and mature rainbow trout (Oncorhynchus mykiss) erythrocytes
Tekijät: Lecklin T, Tuominen A, Nikinmaa M
Kustantaja: COMPANY OF BIOLOGISTS LTD
Julkaisuvuosi: 2000
Journal: Journal of Experimental Biology
Tietokannassa oleva lehden nimi: JOURNAL OF EXPERIMENTAL BIOLOGY
Lehden akronyymi: J EXP BIOL
Vuosikerta: 203
Numero: 19
Aloitussivu: 3025
Lopetussivu: 3031
Sivujen määrä: 7
ISSN: 0022-0949
Tiivistelmä
In this study, we examined whether the adrenergic volume response of teleost erythrocytes is related to cell maturity. Rainbow trout (Oncorhynchus mykiss) were made anaemic by reducing their haematocrit to approximately 50 % of the original value. After 3-4 weeks, small, young erythrocytes were seen in the circulation. By measuring the volume distribution of blood samples from anaemic fish before and after noradrenaline stimulation (10 min, 10(-5) mol l(-1) final concentration), we were able to show that the volume response of young, immature erythrocytes to catecholamine stimulation was greater than that of mature erythrocytes, In addition, the membrane fluidity, measured using the steady-state fluorescence polarisation method, was greater in anaemic fish after 24 days of recovery from bleeding than in control fish. Since blood from anaemic fish contained a large fraction of immature erythrocytes, this result indicates that the fluidity of the membrane of immature erythrocytes is greater than that of mature erythrocytes.
In this study, we examined whether the adrenergic volume response of teleost erythrocytes is related to cell maturity. Rainbow trout (Oncorhynchus mykiss) were made anaemic by reducing their haematocrit to approximately 50 % of the original value. After 3-4 weeks, small, young erythrocytes were seen in the circulation. By measuring the volume distribution of blood samples from anaemic fish before and after noradrenaline stimulation (10 min, 10(-5) mol l(-1) final concentration), we were able to show that the volume response of young, immature erythrocytes to catecholamine stimulation was greater than that of mature erythrocytes, In addition, the membrane fluidity, measured using the steady-state fluorescence polarisation method, was greater in anaemic fish after 24 days of recovery from bleeding than in control fish. Since blood from anaemic fish contained a large fraction of immature erythrocytes, this result indicates that the fluidity of the membrane of immature erythrocytes is greater than that of mature erythrocytes.
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