A1 Refereed original research article in a scientific journal
Novel mutants of Erwinia carotovora subsp carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis
Authors: Laasik E, Ojarand M, Pajunen M, Savilahti H, Mäe A
Publisher: ELSEVIER SCIENCE BV
Publication year: 2005
Journal:: FEMS Microbiology Letters
Journal name in source: FEMS MICROBIOLOGY LETTERS
Journal acronym: FEMS MICROBIOL LETT
Volume: 243
Issue: 1
First page : 93
Last page: 99
Number of pages: 7
ISSN: 0378-1097
DOI: https://doi.org/10.1016/j.femsle.2004.11.045
Abstract
As in Enwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of ca. 10000 clones and screened the clones for the loss of extracellular enzyme production. Extracellular enzymes production was abolished by mutations in the chromosomal hel(Ecc), trkA(Ecc) yheL(Ecc), gls(Ecc), igaA(Ecc) and cysQ(Ecc) genes. The findings reported here demonstrate that we have isolated six new representatives that belong to the pool of genes modulating the production of virulence factors in E. carotovora. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
As in Enwinia carotovora subsp. carotovora the regulation details of the main virulence factors, encoding extracellular enzymes that degrade the plant cell wall, is only rudimentally understood, we performed a genetic screen to identify novel candidate genes involved in the process. Initially, we used Mu transpososome-mediated mutagenesis approach to generate a comprehensive transposon insertion mutant library of ca. 10000 clones and screened the clones for the loss of extracellular enzyme production. Extracellular enzymes production was abolished by mutations in the chromosomal hel(Ecc), trkA(Ecc) yheL(Ecc), gls(Ecc), igaA(Ecc) and cysQ(Ecc) genes. The findings reported here demonstrate that we have isolated six new representatives that belong to the pool of genes modulating the production of virulence factors in E. carotovora. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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