A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Genome characterization of lipid-containing marine bacteriophage PM2 by transposon insertion mutagenesis
Tekijät: Krupovic M, Vilen H, Bamford JKH, Kivelä HM, Aalto JM, Savilahti H, Bamford DH
Kustantaja: AMER SOC MICROBIOLOGY
Julkaisuvuosi: 2006
Lehti: Journal of Virology
Tietokannassa oleva lehden nimi: JOURNAL OF VIROLOGY
Lehden akronyymi: J VIROL
Vuosikerta: 80
Numero: 18
Aloitussivu: 9270
Lopetussivu: 9278
Sivujen määrä: 9
ISSN: 0022-538X
DOI: https://doi.org/10.1128/JVI.00536-06
Tiivistelmä
Bacteriophage PM2 presently is the only member of the Corticoviridae family. The virion consists of a protein-rich lipid vesicle, which is surrounded by an icosahedral protein capsid. The lipid vesicle encloses a supercoiled circular double-stranded DNA genome of 10,079 bp. PM2 belongs to the marine phage community and is known to infect two gram-negative Pseudoalteromonas species. In this study, we present a characterization of the PM2 genome made using the in vitro transposon insertion mutagenesis approach. Analysis of 101 insertion mutants yielded information on the essential and dispensable regions of the PM2 genome and led to the identification of several new genes. A number of lysis-deficient mutants as well as mutants displaying delayed- and/or incomplete-lysis phenotypes were identified. This enabled us to identify novel lysis-associated genes with no resemblance to those previously described from other bacteriophage systems. Nonessential genome regions are discussed in the context of PM2 genome evolution.
Bacteriophage PM2 presently is the only member of the Corticoviridae family. The virion consists of a protein-rich lipid vesicle, which is surrounded by an icosahedral protein capsid. The lipid vesicle encloses a supercoiled circular double-stranded DNA genome of 10,079 bp. PM2 belongs to the marine phage community and is known to infect two gram-negative Pseudoalteromonas species. In this study, we present a characterization of the PM2 genome made using the in vitro transposon insertion mutagenesis approach. Analysis of 101 insertion mutants yielded information on the essential and dispensable regions of the PM2 genome and led to the identification of several new genes. A number of lysis-deficient mutants as well as mutants displaying delayed- and/or incomplete-lysis phenotypes were identified. This enabled us to identify novel lysis-associated genes with no resemblance to those previously described from other bacteriophage systems. Nonessential genome regions are discussed in the context of PM2 genome evolution.
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