A1 Refereed original research article in a scientific journal
A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor
Authors: Rappu P, Shin BS, Zalkin H, Mantsala P
Publisher: AMER SOC MICROBIOLOGY
Publication year: 1999
Journal:: Journal of Bacteriology
Journal name in source: JOURNAL OF BACTERIOLOGY
Journal acronym: J BACTERIOL
Volume: 181
Issue: 12
First page : 3810
Last page: 3815
Number of pages: 6
ISSN: 0021-9193
DOI: https://doi.org/10.1128/JB.181.12.3810-3815.1999
Abstract
Regulation of the purine biosynthetic gene purA was examined by using a transcriptional fusion to a luciferase reporter gene. Transcription was repressed about 10-fold by the addition of adenine and increased approximately 4.5-fold by the addition of guanosine. This regulation is mediated by a purine repressor (PurR), In a purR mutant, basal expression was increased 10-fold, and there was no further stimulation by guanosine or repression by adenine. An open reading frame, yabJ, immediately downstream from purR was found to have a role in the repression of purA by adenine, Repression by adenine was perturbed in a purR(+) yabJ mutant, although guanosine regulation was retained. Mutations in the PurR PRPP binding motif abolished guanosine regulation in the yabJ mutant, Thus, PRPP appears to be required for upregulation by guanosine, The amino acid sequence of YabJ is homologous to the YER057c/YjgF protein family of unknown function.
Regulation of the purine biosynthetic gene purA was examined by using a transcriptional fusion to a luciferase reporter gene. Transcription was repressed about 10-fold by the addition of adenine and increased approximately 4.5-fold by the addition of guanosine. This regulation is mediated by a purine repressor (PurR), In a purR mutant, basal expression was increased 10-fold, and there was no further stimulation by guanosine or repression by adenine. An open reading frame, yabJ, immediately downstream from purR was found to have a role in the repression of purA by adenine, Repression by adenine was perturbed in a purR(+) yabJ mutant, although guanosine regulation was retained. Mutations in the PurR PRPP binding motif abolished guanosine regulation in the yabJ mutant, Thus, PRPP appears to be required for upregulation by guanosine, The amino acid sequence of YabJ is homologous to the YER057c/YjgF protein family of unknown function.
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